Tokarskyy Oleksandr, Marshall Douglas L
Department of Food Science, Nutrition, and Health Promotion, Mississippi Agricultural and Forestry Experiment Station, Mississippi State University, Box 9805, Mississippi State, MS 39762-9805, USA.
Food Microbiol. 2008 Feb;25(1):1-12. doi: 10.1016/j.fm.2007.07.005. Epub 2007 Jul 31.
This review critically evaluates different types of immunosensors proposed for rapid identification of Escherichia coli O157:H7. The methods are compared with approved USDA-FSIS standard procedures for determination of this pathogen in raw or ready-to-eat meat products. Major advantages and disadvantages for each method are highlighted. Our analysis suggests that application of immunosensors in the meat-processing industry may be limited to identification of uncontaminated samples after conventional selective enrichment in broth. Use for detection appears limited at the present time.
本综述批判性地评估了为快速鉴定大肠杆菌O157:H7而提出的不同类型免疫传感器。将这些方法与美国农业部食品安全检验局(USDA-FSIS)批准的用于测定生肉或即食肉类产品中该病原体的标准程序进行了比较。突出了每种方法的主要优缺点。我们的分析表明,免疫传感器在肉类加工业中的应用可能仅限于在肉汤中进行常规选择性富集后对未受污染样品的鉴定。目前其用于检测的用途似乎有限。