Huang Tzu-Wen, Chen Carton W
Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan.
J Bacteriol. 2008 Jan;190(2):755-8. doi: 10.1128/JB.01335-07. Epub 2007 Nov 9.
Both polA (encoding DNA polymerase I; Pol I) and a paralog were deleted from Streptomyces strains. Despite the UV sensitivity and slow growth caused by the DeltapolA mutation, the double mutant was viable. Thus, in contrast to a previous postulate, Pol I and its paralog are not essential for replication of Streptomyces chromosomes.
从链霉菌菌株中删除了polA(编码DNA聚合酶I;Pol I)及其旁系同源物。尽管DeltapolA突变导致了紫外线敏感性和生长缓慢,但双突变体仍可存活。因此,与之前的假设相反,Pol I及其旁系同源物对于链霉菌染色体的复制并非必不可少。