Esteban David J, Syed Aijazuddin, Upton Chris
Department of Biochemistry & Microbiology, University of Victoria.
Methods Mol Biol. 2007;395:187-94. doi: 10.1007/978-1-59745-514-5_11.
In the current genomics era, protein and DNA sequence databases are continuously growing at an exponential rate. It has become increasingly important and useful to repeat similarity searches at frequent intervals, which then retrieve larger and larger sets of results. In addition, sequence similarity searches are now often performed with many sequences or even whole genomes. ReHAB (Recent Hits Acquired from Basic Local Alignment Search Tool [BLAST]) is a tool for tracking new protein hits in repeated PSI-BLAST searches. It is designed to simplify the analysis of large numbers of database matches and is therefore especially suited to comparative genomics. Results are presented in a user-friendly graphical interface with simple-to-navigate tables and new hits are indicated by highlighted text. In this paper, we describe the use of this software for organizing results from whole virus genome PSI-BLAST searches using a ReHAB database maintained at the Virus Bioinformatics Resource Centre.
在当前的基因组学时代，蛋白质和DNA序列数据库正以指数级速度持续增长。频繁进行相似性搜索变得越来越重要且有用，这样能检索到越来越多的结果集。此外，现在序列相似性搜索常常针对许多序列甚至整个基因组进行。ReHAB（从基本局部比对搜索工具[BLAST]获取的近期命中结果）是一种用于在重复的PSI-BLAST搜索中追踪新蛋白质命中结果的工具。它旨在简化对大量数据库匹配结果的分析，因此特别适用于比较基因组学。结果以用户友好的图形界面呈现，带有易于导航的表格，新的命中结果用突出显示的文本表示。在本文中，我们描述了使用该软件来整理来自全病毒基因组PSI-BLAST搜索的结果，这些搜索使用了病毒生物信息学资源中心维护的一个ReHAB数据库。
