Chang Yi-Wei, Yao Hsien-Tsung, Chien Du-Shieng, Yeh Teng-Kuang
Division of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli 350, Taiwan, Republic of China.
Phytochem Anal. 2008 May-Jun;19(3):258-65. doi: 10.1002/pca.1046.
An advanced and reliable HPLC-MS method was developed for the simultaneous quantification of eight active components (ginsenosides Rf, Rg(2), Rg(3), Rh(1) and Rh(2), gomisin A, methylophioponanone B and schizandrin) in Sheng-Mai San, a traditional Chinese medicine. The elution of multiple components was performed using a C(18) column with stepwise gradient elution. The detection of individual analytes was monitored by electrospray MS scanning from 300 to 1000 m/z in the positive ion mode, with the limits of detection of these components ranging from 0.06 to 1 microg/mL at a signal-to-noise ratio of > or =5. The intra- and inter-day accuracies ranged from 95.1 to 104.4%, and the overall precision was less than 9.3%. The recoveries of the analytes were > or =96.6%. The method was validated and found suitable for the determination of active components present in Sheng-Mai San preparation.
建立了一种先进可靠的高效液相色谱-质谱（HPLC-MS）方法，用于同时定量测定中药生脉散中的8种活性成分（人参皂苷Rf、Rg(2)、Rg(3)、Rh(1)和Rh(2)、五味子醇甲、甲基五叶参醌B和五味子素）。使用C(18)柱通过梯度洗脱进行多种成分的洗脱。在正离子模式下，通过电喷雾质谱扫描在300至1000 m/z范围内监测各个分析物的检测，这些成分的检测限在信噪比≥5时为0.06至1μg/mL。日内和日间准确度范围为95.1%至104.4%，总体精密度小于9.3%。分析物的回收率≥96.6%。该方法经过验证，适用于生脉散制剂中活性成分的测定。
