蛋白质在聚乙烯醇水凝胶表面的共价固定化。

Covalent immobilization of proteins on to the surface of poly(vinyl alcohol) hydrogel.

作者信息

Kobayashi H, Ikada Y

机构信息

Research Center for Biomedical Engineering, Kyoto University, Japan.

出版信息

Biomaterials. 1991 Oct;12(8):747-51. doi: 10.1016/0142-9612(91)90024-5.

DOI:10.1016/0142-9612(91)90024-5

PMID:1799650

Abstract

Covalent immobilization of cell-adhesive proteins such as collagen and fibronectin on to the surface of poly(vinyl alcohol) hydrogel was investigated by using diisocyanates, polyisocyanates, and cyanogen bromide. It was found that 0.5 and 12 micrograms/cm2 of collagen were immobilized on to the surface by using hexamethylene diisocyanate and cyanogen bromide, respectively. The big difference in the graft amount between the two methods was ascribed to the different reaction media employed for the surface modifications; toluene for the reaction with hexamethylene diisocyanate and water for the reaction with cyanogen bromide.

摘要

通过使用二异氰酸酯、多异氰酸酯和溴化氰，研究了将胶原蛋白和纤连蛋白等细胞粘附蛋白共价固定到聚乙烯醇水凝胶表面的方法。结果发现，分别使用六亚甲基二异氰酸酯和溴化氰时，每平方厘米表面分别固定了0.5微克和12微克的胶原蛋白。两种方法在接枝量上的巨大差异归因于表面改性所采用的不同反应介质；与六亚甲基二异氰酸酯反应使用甲苯，与溴化氰反应使用水。