Jia Lewen, Wang Chang, Zhao Sumin, Lu Xin, Xu Guowang
National Chromatographic R&A Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116023, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Dec 1;860(1):134-40. doi: 10.1016/j.jchromb.2007.10.033. Epub 2007 Nov 8.
Plasma phospholipids metabolic profile of chronic glomerulonephritis was investigated using high performance liquid chromatography/mass spectrometry (LC/MS) and principal component analysis. The plasma samples of 18 patients with chronic glomerulonephritis, 17 patients with chronic renal failure (CRF) without renal replacement therapy and 18 healthy persons were collected and analyzed. It was found that combination of the LC/MS technique with PCA can be successfully applied to phospholipid profile analysis. The results showed that primary chronic glomerulonephritis and CRF had phospholipids metabolic abnormality. Nineteen phospholipid species were identified as possible biomarkers in plasma samples of chronic glomerulonephritis and chronic renal failure. Moreover, the identification of the molecular structure of the potential phospholipid markers was obtained by ESI-MS/MS experiment. It suggests that phospholipids can be used as potential biomarkers on the progress of primary chronic glomerulonephritis.
采用高效液相色谱/质谱联用技术（LC/MS）和主成分分析法，对慢性肾小球肾炎患者的血浆磷脂代谢谱进行了研究。收集并分析了18例慢性肾小球肾炎患者、17例未接受肾脏替代治疗的慢性肾衰竭（CRF）患者以及18例健康人的血浆样本。结果发现，LC/MS技术与主成分分析法相结合可成功应用于磷脂谱分析。结果显示，原发性慢性肾小球肾炎和慢性肾衰竭存在磷脂代谢异常。在慢性肾小球肾炎和慢性肾衰竭患者的血浆样本中，鉴定出19种磷脂种类可能作为生物标志物。此外，通过电喷雾串联质谱（ESI-MS/MS）实验，对潜在磷脂标志物的分子结构进行了鉴定。这表明磷脂可作为原发性慢性肾小球肾炎进展的潜在生物标志物。
