Department of Gerontology, Shengjing Affiliated Hospital, China Medical University, Shenyang 110004, PR China.
Clin Biochem. 2010 Aug;43(12):992-7. doi: 10.1016/j.clinbiochem.2010.04.072. Epub 2010 May 10.
The identification of Alzheimer's disease (AD) biomarkers may allow for a less invasive and more accurate diagnosis as well as serve as a predictor of future disease progression and treatment response. The aim of this study was to map potential biomarkers in plasma for AD.
Plasma metabolic perturbations between AD and healthy old person were investigated using ultra performance liquid chromatography/mass spectrometry (UPLC/MS) and metabonomics approach. The principal component analysis (PCA) of UPLC/MS spectra showed that metabolic changes between two groups.
The PCA of UPLC/MS spectra showed that metabolic changes observed between AD and control were clear. Nine potential biomarkers in correlation with the extent of AD were found.
Based on PCA, several potential biomarkers (LPCs, sphingosine and tryptophan) were found and further identified by the following LC/MS/MS analysis. All of them could be the potential early markers of AD.
阿尔茨海默病（AD）生物标志物的鉴定可以实现更具侵袭性和更准确的诊断，并且可以作为未来疾病进展和治疗反应的预测指标。本研究旨在绘制 AD 血浆中潜在的生物标志物图谱。
采用超高效液相色谱/质谱联用（UPLC/MS）和代谢组学方法研究 AD 与健康老年人之间的血浆代谢紊乱。UPLC/MS 谱的主成分分析（PCA）显示两组之间存在代谢变化。
UPLC/MS 谱的 PCA 显示 AD 与对照组之间的代谢变化明显。发现了 9 个与 AD 程度相关的潜在生物标志物。
基于 PCA，通过以下 LC/MS/MS 分析进一步鉴定了几个潜在的生物标志物（LPC、神经酰胺和色氨酸）。它们都可能是 AD 的潜在早期标志物。
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