Thomson L A, Law F C, James K H, Rushton N
University of Cambridge Orthopaedic Research Unit, Addenbrooke's Hospital, UK.
Biomaterials. 1991 Oct;12(8):781-5. doi: 10.1016/0142-9612(91)90030-e.
This research was to determine the biocompatibility of simulated wear particles from a GORE-TEX cruciate ligament prosthesis using in vitro and in vivo techniques. Exposure of normal human synovial fibroblasts and primary mouse peritoneal macrophages to the particles revealed no cytotoxicity, as determined by lactate dehydrogenase release, but beta-N-acetyl-D-glucosaminidase was released from macrophages at high concentrations, showing inflammatory potential. This was not seen when the particles were injected into mouse knees, where no adverse reactions were observed. These simulated wear particles were shown to be biocompatible using these in vitro and in vivo systems.
本研究旨在采用体外和体内技术，确定聚四氟乙烯人工交叉韧带假体模拟磨损颗粒的生物相容性。通过乳酸脱氢酶释放测定，将正常人滑膜成纤维细胞和原代小鼠腹腔巨噬细胞暴露于这些颗粒中未显示出细胞毒性，但在高浓度下巨噬细胞会释放β-N-乙酰-D-氨基葡萄糖苷酶，显示出炎症潜能。当将这些颗粒注射到小鼠膝关节时未观察到这种情况，也未观察到不良反应。使用这些体外和体内系统表明，这些模拟磨损颗粒具有生物相容性。
