Ferguson J M, MacGregor I R, McLaughlin L F, Prowse C V
Wellcome Surgical Institute, University of Glasgow.
Blood Coagul Fibrinolysis. 1991 Dec;2(6):731-4. doi: 10.1097/00001721-199112000-00006.
Artificial insemination with frozen semen was used to breed beagles homozygous for a deficiency in clotting factor VII, with plasma concentrations of 2-3% of normal adult beagle pooled plasma. The factor VII-deficient beagles were used to determine if factor VII activity has a role in the thrombogenic activity of prothrombin complex concentrates (PCCs) containing factors II, IX and X by evaluating changes in selected indicators of thrombogenicity. No differences were seen between the responsiveness of the factor VII-deficient and normal beagles, indicating that the thrombogenic activities of PCCs are mediated via effects on the intrinsic clotting system.
采用冷冻精液人工授精的方法繁育了凝血因子 VII 缺乏的纯合比格犬，其血浆浓度为正常成年比格犬混合血浆的 2%-3%。通过评估血栓形成性的选定指标变化，利用这些凝血因子 VII 缺乏的比格犬来确定因子 VII 活性在含有因子 II、IX 和 X 的凝血酶原复合物浓缩物（PCCs）的血栓形成活性中是否起作用。凝血因子 VII 缺乏的比格犬和正常比格犬的反应性未见差异，这表明 PCCs 的血栓形成活性是通过对内源性凝血系统的作用介导的。
