Robles Ana, Clayton Christine
Zentrum für Molekulare Biologie (ZMBH), Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany.
Mol Biochem Parasitol. 2008 Jan;157(1):102-6. doi: 10.1016/j.molbiopara.2007.09.005. Epub 2007 Oct 2.
Trypanosoma brucei regulates gene expression by post-transcriptional mechanisms, such as mRNA turnover and translation control. This regulation frequently requires specific sequences located in the 3'-untranslated region. Microarray analysis and Northern blot hybridization showed that the amino acid transporter 11 mRNA is up-regulated in insect stages of the parasite. By RT-PCR and sequencing, the AATP11 polyadenylation site was mapped. We show that this 3'-UTR causes higher expression of the chloramphenicol acetyltransferase (CAT) reporter gene in procyclic trypanosomes than in bloodstream forms. Results of deletion analysis suggested that multiple elements located between nucleotides 141 and 618 of the 3'-untranslated region are required for this control.
布氏锥虫通过转录后机制（如mRNA周转和翻译控制）来调节基因表达。这种调节通常需要位于3'-非翻译区的特定序列。微阵列分析和Northern印迹杂交表明，氨基酸转运蛋白11 mRNA在寄生虫的昆虫阶段上调。通过RT-PCR和测序，定位了AATP11聚腺苷酸化位点。我们发现，与血流形式的锥虫相比，该3'-UTR在原循环锥虫中使氯霉素乙酰转移酶（CAT）报告基因的表达更高。缺失分析结果表明，3'-非翻译区核苷酸141和618之间的多个元件是这种调控所必需的。
