Sugaya Kimio, Nishijima Saori, Tasaki Shinsuke, Kadekawa Katsumi, Miyazato Minoru, Ogawa Yoshihide
Division of Urology, Department of Organ-oriented Medicine, Faculty of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, Japan.
Neurosci Lett. 2007 Dec 18;429(2-3):142-6. doi: 10.1016/j.neulet.2007.10.003. Epub 2007 Oct 18.
We examined the effects of propiverine hydrochloride and naftopidil on the urinary ATP level and bladder activity after bladder stimulation in rats. Thirty-nine female rats were divided into a control group, a propiverine group, and a naftopidil group. Rats in the propiverine and naftopidil groups were administered 1 ml/day of propiverine or naftopidil dissolved in water at 5mg/ml, while animals in the control group were administered 1 ml of water only. After 2 weeks, 18 rats (6 per group) underwent continuous cystometry with physiological saline or 0.1% acetic acid solution and their bladder activity was recorded. In the remaining 21 rats (7 per group), 0.1% acetic acid solution was infused into the bladder and the urinary ATP level was measured before and after stimulation (days 0-7). During cystometry with acetic acid in the control group, the interval between bladder contractions was shorter and the maximum bladder contraction pressure was higher than the results for cystometry with physiological saline. The maximum bladder contraction pressure was also increased in the naftopidil group, but such a change was not seen in the propiverine group. The urinary ATP level increased significantly in all three groups after the infusion of acetic acid, but the increase of ATP was smaller in the propiverine group (45% of control) and the naftopidil group (62% of control) than in the control group on day 0. Therefore, the inhibitory effect of propiverine and naftopidil on bladder activity may be partly due to blocking ATP release from the bladder epithelium.
我们研究了盐酸丙哌维林和萘哌地尔对大鼠膀胱刺激后尿ATP水平及膀胱活动的影响。39只雌性大鼠被分为对照组、丙哌维林组和萘哌地尔组。丙哌维林组和萘哌地尔组的大鼠每天给予1 ml溶解于水中的丙哌维林或萘哌地尔（浓度为5mg/ml），而对照组的动物仅给予1 ml水。2周后，18只大鼠（每组6只）用生理盐水或0.1%醋酸溶液进行连续膀胱测压，并记录其膀胱活动。在其余21只大鼠（每组7只）中，向膀胱内注入0.1%醋酸溶液，并在刺激前后（第0 - 7天）测量尿ATP水平。在对照组用醋酸进行膀胱测压期间，膀胱收缩间隔时间较短，最大膀胱收缩压力高于用生理盐水进行膀胱测压的结果。萘哌地尔组的最大膀胱收缩压力也有所增加，但丙哌维林组未见此变化。注入醋酸后，所有三组的尿ATP水平均显著升高，但丙哌维林组（为对照组的45%）和萘哌地尔组（为对照组的62%）的ATP增加幅度小于第0天的对照组。因此，丙哌维林和萘哌地尔对膀胱活动的抑制作用可能部分归因于阻断膀胱上皮细胞释放ATP。
