抗农达玉米和大豆的重组DNA在轮作中的定量分析与持久性

Quantification and persistence of recombinant DNA of Roundup Ready corn and soybean in rotation.

作者信息

Lerat Sylvain, Gulden Robert H, Hart Miranda M, Powell Jeff R, England Laura S, Pauls K Peter, Swanton Clarence J, Klironomos John N, Trevors Jack T

机构信息

Departments of Environmental Biology, Plant Agriculture, and Integrative Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1.

出版信息

J Agric Food Chem. 2007 Dec 12;55(25):10226-31. doi: 10.1021/jf072457z. Epub 2007 Nov 13.

DOI:10.1021/jf072457z

PMID:17997522

Abstract

The presence of the recombinant cp4 epsps gene from Roundup Ready (RR) corn and RR soybean was quantified using real-time PCR in soil samples from a field experiment growing RR and conventional corn and soybean in rotation. RR corn and RR soybean cp4 epsps persisted in soil for up to 1 year after seeding. The concentration of recombinant DNA in soil peaked in July and August in RR corn and RR soybean plots, respectively. A small fraction of soil samples from plots seeded with conventional crops contained recombinant DNA, suggesting transgene dispersal by means of natural process or agricultural practices. This research will aid in the understanding of the persistence of recombinant DNA in agricultural cropping systems.

摘要

利用实时荧光定量PCR技术，对一块轮作种植抗草甘膦（RR）玉米和RR大豆以及常规玉米和大豆的田间试验土壤样本中，来自RR玉米和RR大豆的重组cp4 epsps基因的存在情况进行了定量分析。RR玉米和RR大豆中的cp4 epsps在播种后在土壤中可存留长达1年。RR玉米和RR大豆地块土壤中重组DNA的浓度分别在7月和8月达到峰值。一些种植常规作物地块的土壤样本中含有重组DNA，这表明转基因可通过自然过程或农业操作进行扩散。该研究将有助于了解农业种植系统中重组DNA的存留情况。