Zhang Jie-mei, Guo Jie, Tu Xin, Shi Zhao-hong, Hao Jian-jun, Ke Yu-he, Guan Jiang-feng, He Juan-juan
Unit of Cardiology, Institute of Integrative Medicine, Wuhan First Hospital, and Human Genome Research Centre, Life and Technology College, Huazhong University of Science and Technology, Wuhan, Hubei Province 430022, China.
Zhong Xi Yi Jie He Xue Bao. 2007 Nov;5(6):675-80. doi: 10.3736/jcim20070615.
To observe the protective effect of Huaxia shallot preparation on human umbilical vein endothelial cell (HUVEC) injury induced by oxidized low density lipoprotein (Ox-LDL) in vitro.
Ox-LDL was prepared and identified, and HUVECs were cultured. After 2-hour intervention of different drugs and 24-hour following intervention of Ox-LDL, the number of HUVECs was observed by phase contrast optical microscope and the activity of the HUVECs was observed by methyl thiazolyl tetrazolium (MTT) technique. Superoxide dismutase (SOD) activity and nitric oxide (NO) content were assayed by respective kit. The protein expressions and mRNA levels of peroxisome proliferators activated receptor gamma(PPAR-gamma) and endothelial nitric oxide synthase (eNOS) were measured by western blot technique and reverse transcription polymerase chain reaction (RT-PCR).
Ox-LDL could increase the apoptosis rate of the HUVECs and decrease the NO release as compared with the blank control group (P<0.05). These effects induced by Ox-LDL were all significantly inhibited by Huaxia shallot preparation. It could up-regulate the protein expressions and mRNA levels of PPAR-gamma and eNOS significantly (P<0.05). Huaxia shallot preparation could decrease the apoptosis rate of the HUVECs.
Ox-LDL may be involved in the initiation and progression of atherosclerosis by injuring the endothelial cells directly and may cause the endothelial dysfunction. Huaxia shallot preparation can protect against Ox-LDL induced endothelial cell injury by up-regulating the protein expressions and mRNA levels of PPAR-gamma and eNOS. It suggests that Huaxia shallot preparation may play a role in the prevention and treatment of cardiovascular disease.
观察华夏葱制剂对体外氧化型低密度脂蛋白（Ox-LDL）诱导的人脐静脉内皮细胞（HUVEC）损伤的保护作用。
制备并鉴定Ox-LDL，培养HUVEC。不同药物干预2小时后，再用Ox-LDL干预24小时，用相差光学显微镜观察HUVEC数量，用噻唑蓝（MTT）法观察HUVEC活性。用相应试剂盒检测超氧化物歧化酶（SOD）活性和一氧化氮（NO）含量。采用蛋白质印迹法和逆转录聚合酶链反应（RT-PCR）检测过氧化物酶体增殖物激活受体γ（PPAR-γ）和内皮型一氧化氮合酶（eNOS）的蛋白表达及mRNA水平。
与空白对照组相比，Ox-LDL可增加HUVEC的凋亡率，降低NO释放（P<0.05）。华夏葱制剂可显著抑制Ox-LDL诱导的这些效应。它可显著上调PPAR-γ和eNOS的蛋白表达及mRNA水平（P<0.05）。华夏葱制剂可降低HUVEC的凋亡率。
Ox-LDL可能通过直接损伤内皮细胞参与动脉粥样硬化的发生发展，并可能导致内皮功能障碍。华夏葱制剂可通过上调PPAR-γ和eNOS的蛋白表达及mRNA水平，保护内皮细胞免受Ox-LDL诱导的损伤。提示华夏葱制剂可能在心血管疾病的防治中发挥作用。
