复方水蛭滴眼液对大鼠晶状体上皮细胞凋亡及Bcl-2和Bax基因表达的抑制作用

[Inhibition effects of compound leech eye drops on apoptosis of lens epithelial cells and expressions of Bcl-2 and Bax genes in rats].

作者信息

Huang Xiu-rong, Qi Ming-xin, Wang Zhao-yang, Chen Yi

机构信息

Research Center of Pathophysiology, Fujian College of Traditional Chinese Medicine, Fuzhou, Fujian Province 350003, China.

出版信息

Zhong Xi Yi Jie He Xue Bao. 2007 Nov;5(6):681-5. doi: 10.3736/jcim20070616.

DOI:10.3736/jcim20070616

Abstract

OBJECTIVE

To investigate the inhibition effects of compound leech eye drops (Co-SZ) on apoptosis of lens epithelial cells (LECs) induced by hydrogen peroxide (H2O2) and the expressions of apoptosis-related genes Bcl-2 and Bax in rats.

METHODS

All fresh transparent LECs in SD rats were bathed in culture medium with H2O2 in vitro, meanwhile Co-SZ were added in the culture medium. All LECs were incubated for 24 hours. The apoptosis rate of LECs was determined by terminal deoxynucleotidyl transferase mediated biotin-dUTP nick end labeling method (TUNEL). The changes of LEC ultrastructure and the formation of apoptotic body were observed by transmission electron microscopy. The expressions of apoptosis-related genes Bcl-2 and Bax were detected by streptavdin-peroxidase-biotin method.

RESULTS

The apoptosis rate of LECs in the Co-SZ-treated group was significantly lower than that in the H2O2-treated group. The changes of apoptotic LEC ultrastructure in the Co-SZ-treated group were less than those in the H2O2-treated group. The expression of Bcl-2 protein was up-regulated and the expression of Bax protein was down-regulated in the Co-SZ-treated group as compared with the H2O2-treated group.

CONCLUSION

The LEC apoptosis induced by H2O2 can be inhibited by Co-SZ. The molecular mechanisms of Co-SZ in inhibiting LEC apoptosis may be related to regulating the expressions of apoptosis-related genes Bcl-2 and Bax.

摘要

目的

探讨复方水蛭滴眼液（Co-SZ）对过氧化氢（H2O2）诱导的大鼠晶状体上皮细胞（LECs）凋亡及凋亡相关基因Bcl-2和Bax表达的抑制作用。

方法

将SD大鼠所有新鲜透明的LECs在体外置于含H2O2的培养基中培养，同时在培养基中加入Co-SZ。所有LECs孵育24小时。采用末端脱氧核苷酸转移酶介导的生物素-dUTP缺口末端标记法（TUNEL）测定LECs的凋亡率。通过透射电子显微镜观察LECs超微结构的变化及凋亡小体的形成。采用链霉亲和素-过氧化物酶-生物素法检测凋亡相关基因Bcl-2和Bax的表达。

结果

Co-SZ处理组LECs的凋亡率显著低于H2O2处理组。Co-SZ处理组凋亡LECs超微结构的变化小于H2O2处理组。与H2O2处理组相比，Co-SZ处理组Bcl-2蛋白表达上调，Bax蛋白表达下调。

结论

Co-SZ可抑制H2O2诱导的LECs凋亡。Co-SZ抑制LECs凋亡的分子机制可能与调节凋亡相关基因Bcl-2和Bax的表达有关。