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本文引用的文献

1
FastSPECT II: A Second-Generation High-Resolution Dynamic SPECT Imager.快速单光子发射计算机断层扫描II：第二代高分辨率动态单光子发射计算机断层扫描仪
IEEE Trans Nucl Sci. 2004 Jun;51(3):631-635. doi: 10.1109/TNS.2004.830975.
2
Imaging acute cardiac cell death: temporal and spatial distribution of 99mTc-labeled C2A in the area at risk after myocardial ischemia and reperfusion.急性心肌细胞死亡的成像：心肌缺血再灌注后危险区域内99mTc标记的C2A的时间和空间分布。
J Nucl Med. 2007 Jun;48(6):1031-6. doi: 10.2967/jnumed.106.037754.
3
A paramagnetic nanoprobe to detect tumor cell death using magnetic resonance imaging.一种用于通过磁共振成像检测肿瘤细胞死亡的顺磁性纳米探针。
Nano Lett. 2007 May;7(5):1419-23. doi: 10.1021/nl070126v. Epub 2007 Apr 6.
4
Noninvasive detection of programmed cell loss with 99mTc-labeled annexin A5 in heart failure.用99mTc标记的膜联蛋白A5对心力衰竭中程序性细胞丢失进行无创检测。
J Nucl Med. 2007 Apr;48(4):562-7. doi: 10.2967/jnumed.106.039453.
5
99mTc-labeled C2A domain of synaptotagmin I as a target-specific molecular probe for noninvasive imaging of acute myocardial infarction.99mTc标记的突触结合蛋白I的C2A结构域作为急性心肌梗死无创成像的靶向特异性分子探针。
J Nucl Med. 2006 Aug;47(8):1367-74.
6
99mTc-labeled annexin V fragments: a potential SPECT radiopharmaceutical for imaging cell death.99mTc标记的膜联蛋白V片段：一种用于细胞死亡成像的潜在单光子发射计算机断层显像放射性药物。
Nucl Med Biol. 2006 Jul;33(5):635-43. doi: 10.1016/j.nucmedbio.2006.05.002.
7
Systemic inflammation and reperfusion injury in patients with acute myocardial infarction.急性心肌梗死患者的全身炎症与再灌注损伤
Mediators Inflamm. 2005 Dec 14;2005(6):385-9. doi: 10.1155/MI.2005.385.
8
(99m)Tc-annexin V and (111)In-antimyosin antibody uptake in experimental myocardial infarction in rats.大鼠实验性心肌梗死中（99m）锝标记的膜联蛋白V和（111）铟标记的抗肌凝蛋白抗体摄取情况
Eur J Nucl Med Mol Imaging. 2006 Mar;33(3):239-45. doi: 10.1007/s00259-005-1900-2. Epub 2005 Nov 10.
9
Detection of apoptosis using the C2A domain of synaptotagmin I.使用突触结合蛋白I的C2A结构域检测细胞凋亡。
Bioconjug Chem. 2004 Sep-Oct;15(5):983-7. doi: 10.1021/bc049899q.
10
High-resolution imaging with (99m)Tc-glucarate for assessing myocardial injury in rat heart models exposed to different durations of ischemia with reperfusion.使用（99m）Tc-葡萄糖醛酸进行高分辨率成像，以评估在经历不同时长缺血再灌注的大鼠心脏模型中的心肌损伤。
J Nucl Med. 2004 Jul;45(7):1251-9.

在大鼠缺血再灌注模型中，使用99mTc标记的突触结合蛋白I的C2A结构域对心肌细胞死亡进行体内动态成像。

In vivo dynamic imaging of myocardial cell death using 99mTc-labeled C2A domain of synaptotagmin I in a rat model of ischemia and reperfusion.

作者信息

Liu Zhonglin, Zhao Ming, Zhu Xiaoguang, Furenlid Lars R, Chen Yi-Chun, Barrett Harrison H

机构信息

Department of Radiology, University of Arizona, Tucson, AZ 85724, USA.

出版信息

Nucl Med Biol. 2007 Nov;34(8):907-15. doi: 10.1016/j.nucmedbio.2007.07.013. Epub 2007 Sep 19.

DOI:10.1016/j.nucmedbio.2007.07.013
PMID:17998092
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2515710/
Abstract

OBJECTIVES

This study was designed to investigate the capability of a small-animal SPECT imager, FastSPECT II, for dynamic rat heart imaging and to characterize the in vivo kinetic properties of 99mTc-C2A-glutathione-s-transferase (GST), a molecular probe targeting apoptosis and necrosis, in detecting cell death in ischemic-reperfused rat hearts.

METHODS

C2A-GST was radiolabeled with 99mTc via 2-iminothiolane thiolation. Myocardial ischemia-reperfusion was induced by 30-min ligation of the left coronary artery followed by 120-min reperfusion in seven rats. FastSPECT II cardiac images of 99mTc-C2A-GST in list-mode acquisition were recorded for 2 h using FastSPECT II.

RESULTS

Tomographic images showed a focal radioactive accumulation (hot spot) in the lateral and anterior walls of the left ventricle. The hot spot was initially visualized 10 min after injection and persisted on the 2-h images. Quantitative analysis demonstrated that the hot-spot radioactivity increased significantly within 30 min postinjection and experienced no washout up to the end of the 2-h study. The ratio of the hot spot/viable myocardium was 4.52+/-0.24, and infarct-to-lung ratio was 8.22+/-0.63 at 2 h postinjection. The uptake of 99mTc-C2A-GST in the infarcted myocardium was confirmed by triphenyl tetrazolium chloride staining and autoradiography analysis.

CONCLUSIONS

FastSPECT II allows quantitative dynamic imaging and functional determination of radiotracer kinetics in rat hearts. An in vivo kinetic profile of 99mTc-C2A-GST in the ischemic-reperfused rat heart model was characterized successfully. The pattern of accelerated 99mTc-C2A-GST uptake in the ischemic area at risk after reperfusion may be useful in detecting and quantifying ongoing myocardial cell loss induced by ischemia-reperfusion.

摘要

目的

本研究旨在探讨小动物单光子发射计算机断层显像仪（SPECT）FastSPECT II对大鼠心脏进行动态成像的能力，并表征99mTc-C2A-谷胱甘肽-S-转移酶（GST）（一种靶向凋亡和坏死的分子探针）在检测缺血再灌注大鼠心脏细胞死亡中的体内动力学特性。

方法

通过2-亚氨基硫烷硫醇化反应，用99mTc对C2A-GST进行放射性标记。在7只大鼠中，通过结扎左冠状动脉30分钟，然后再灌注120分钟诱导心肌缺血再灌注。使用FastSPECT II以列表模式采集99mTc-C2A-GST的心脏图像，记录2小时。

结果

断层图像显示左心室侧壁和前壁有局灶性放射性聚集（热点）。注射后10分钟开始出现热点，并在2小时图像上持续存在。定量分析表明，注射后30分钟内热点放射性显著增加，直至2小时研究结束均无洗脱。注射后2小时，热点/存活心肌的比值为4.52±0.24，梗死/肺比值为8.22±0.63。通过氯化三苯基四氮唑染色和放射自显影分析证实了梗死心肌中99mTc-C2A-GST的摄取。

结论

FastSPECT II可对大鼠心脏进行定量动态成像和放射性示踪剂动力学的功能测定。成功表征了99mTc-C2A-GST在缺血再灌注大鼠心脏模型中的体内动力学特征。再灌注后缺血危险区域99mTc-C2A-GST摄取加速的模式可能有助于检测和量化缺血再灌注引起的持续性心肌细胞丢失。