Weber T E, Kerr B J
USDA-ARS, National Swine Research and Information Center, Ames, IA 50011-3310, USA.
J Anim Sci. 2008 Feb;86(2):442-50. doi: 10.2527/jas.2007-0499. Epub 2007 Nov 12.
Two experiments were conducted to determine the effects of dietary sodium butyrate on growth performance and response to Escherichia coli lipopolysaccharide (LPS) in weanling pigs. In a 28-d experiment, 180 pigs (initial BW 6.3 kg) were fed 0, 0.05, 0.1, 0.2, or 0.4% sodium butyrate, or 110 mg/kg of dietary tylosin. There was no effect of dietary sodium butyrate or tylosin on overall G:F, but there was a linear trend (P < 0.07) toward decreased ADFI and ADG as levels of sodium butyrate increased. In a second 28-d experiment, 108 pigs (initial BW 6.3 kg) were assigned to 1 of 3 dietary treatments: 1) no antibiotics, 2) 0.2% sodium butyrate, or 3) 55 mg/kg of carbadox. On d 14, a subset of pigs from the no-antibiotic and butyrate treatment groups was challenged with E. coli LPS or injected with sterile saline in a 2 x 2 factorial arrangement (+/-LPS challenge; +/-dietary butyrate; n = 6 pigs/treatment group). Four hours after LPS challenge, blood samples were obtained, and samples of LM, liver, and ileum were collected for gene expression analysis. Serum samples were analyzed for IL-6, tumor necrosis factor alpha (TNFalpha), alpha(1)-acid glycoprotein, cortisol, IGF-I, insulin, and metabolites. The relative abundance of tissue cytokine and IGF-I mRNA was measured by real-time PCR. Feeding diets containing sodium butyrate or carbadox did not alter ADG or ADFI compared with pigs fed the control diet. From d 0 to 14, pigs fed diets containing 0.2% sodium butyrate had decreased (P < 0.05) ADG and tended (P < 0.06) to have decreased G:F compared with animals fed diets containing carbadox. Challenge with LPS increased (P < 0.05) serum cytokines and cortisol and decreased (P < 0.05) serum glucose and triglycerides. Injection with LPS increased (P < 0.05) the relative abundance of hepatic IL-6 and TNFalpha mRNA, increased (P < 0.05) LM TNFalpha mRNA content, and decreased (P < 0.05) IGF-I mRNA in LM. For serum cortisol, there was an interaction (P < 0.05) between dietary butyrate and LPS. The increase in serum cortisol attributable to LPS was greater (P < 0.05) in pigs fed butyrate than in pigs fed the control diet. There tended (P < 0.10) to be an interaction between LPS and diet and for butyrate to increase the relative abundance of IL-6 mRNA in LM. Carbadox did not alter cytokine or IGF-I mRNA or serum metabolites, but did decrease (P < 0.05) serum TNFalpha. These data indicate that dietary sodium butyrate does not enhance growth performance, but may regulate the response to inflammatory stimuli in weanling pigs.
进行了两项试验，以确定日粮中添加丁酸钠对断奶仔猪生长性能和对大肠杆菌脂多糖（LPS）反应的影响。在一项为期28天的试验中，180头仔猪（初始体重6.3千克）分别饲喂含0、0.05%、0.1%、0.2%或0.4%丁酸钠的日粮，或110毫克/千克的泰乐菌素。日粮中添加丁酸钠或泰乐菌素对总生长育肥比（G:F）没有影响，但随着丁酸钠水平的增加，平均日采食量（ADFI）和平均日增重（ADG）呈线性下降趋势（P<0.07）。在第二项为期28天的试验中，108头仔猪（初始体重6.3千克）被分配到3种日粮处理中的一种：1）不添加抗生素，2）添加0.2%丁酸钠，或3）添加55毫克/千克的卡巴氧。在第14天，来自不添加抗生素和丁酸钠处理组的一部分仔猪按照2×2析因设计（±LPS攻毒；±日粮丁酸钠；每组n=6头猪）接受大肠杆菌LPS攻毒或注射无菌生理盐水。LPS攻毒4小时后，采集血样，并采集腰大肌（LM）、肝脏和回肠样本用于基因表达分析。分析血清样本中的白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNFα）、α1-酸性糖蛋白、皮质醇、胰岛素样生长因子-I（IGF-I）、胰岛素和代谢产物。通过实时荧光定量PCR测定组织细胞因子和IGF-I mRNA的相对丰度。与饲喂对照日粮的仔猪相比，饲喂含丁酸钠或卡巴氧日粮的仔猪ADG和ADFI没有改变。从第0天到第14天，与饲喂含卡巴氧日粮的仔猪相比，饲喂含0.2%丁酸钠日粮的仔猪ADG降低（P<0.05），G:F有降低趋势（P<0.06）。LPS攻毒使血清细胞因子和皮质醇增加（P<0.05），血清葡萄糖和甘油三酯降低（P<0.05）。注射LPS使肝脏IL-6和TNFα mRNA的相对丰度增加（P<0.05），LM中TNFα mRNA含量增加（P<0.05），LM中IGF-I mRNA降低（P<0.05）。对于血清皮质醇，日粮丁酸钠和LPS之间存在交互作用（P<0.05）。LPS引起的血清皮质醇增加在饲喂丁酸钠的仔猪中比饲喂对照日粮的仔猪更大（P<0.05）。LPS和日粮之间有交互作用的趋势（P<0.10），丁酸钠使LM中IL-6 mRNA的相对丰度增加。卡巴氧不改变细胞因子或IGF-I mRNA或血清代谢产物，但使血清TNFα降低（P<0.05）。这些数据表明，日粮丁酸钠不能提高生长性能，但可能调节断奶仔猪对炎症刺激的反应。
