Dyomina E A, Ryabchenko N M
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine.
Exp Oncol. 2007 Sep;29(3):217-20.
Evaluation of chromosomal radiosensitivity of healthy individuals and determination those with the increased susceptibility to radiogenic cancer.
Cytogenetic examination of radiation induced injuries in lymphocytes of healthy individuals (n=103) was carried out on the basis of G(2)-assay. Test system of peripheral blood lymphocytes with metaphase analysis was used.
On the basis of the obtained "stage-effect" and "dose-effect" calibrating curves the scheme of cytogenetic examinations of healthy individuals was developed. Analysis of cytogenetic parameters induced by G(2) irradiation at 1.5 Gy dose revealed their high interindividual variability. The highest differences were registered for chromatid type aberrations (CV=42.1%) with the chromatid break predominance in the spectrum (CV=37.5%). Statistical analysis of the distributions of the obtained individual cytogenetic parameters indicated 12% individuals with increased chromosomal radiosensitivity.
Cytogenetic evaluation of individual chromosomal radiosensitivity based on G(2)-assay has its perspectives in the formation of groups with increased risk of radiogenic cancer developing and its primary prophylactics among healthy population.
评估健康个体的染色体放射敏感性，并确定那些对辐射诱发癌症易感性增加的个体。
基于G(2)检测法，对103名健康个体的淋巴细胞辐射诱导损伤进行细胞遗传学检查。采用外周血淋巴细胞中期分析测试系统。
根据获得的“阶段效应”和“剂量效应”校准曲线，制定了健康个体细胞遗传学检查方案。对1.5 Gy剂量的G(2)照射诱导的细胞遗传学参数分析显示，它们具有高度的个体间变异性。染色单体型畸变差异最大（变异系数=42.1%），且染色单体断裂在谱中占主导（变异系数=37.5%）。对所获得的个体细胞遗传学参数分布进行统计分析表明，12%的个体染色体放射敏感性增加。
基于G(2)检测法的个体染色体放射敏感性细胞遗传学评估，在确定辐射诱发癌症风险增加的群体及其在健康人群中的一级预防方面具有前景。
