采用高效液相色谱-电化学检测法测定皮摩尔量的脂氧素C4、D4和E4。

Determination of picomole amounts of lipoxins C4, D4 and E4 by high-performance liquid chromatography with electrochemical detection.

作者信息

Gaede F P, Kirchner M, Steinhilber D, Roth H J

机构信息

Pharmaceutical Institute, University of Tübingen, Germany.

出版信息

J Chromatogr. 1991 Oct 25;585(1):53-6. doi: 10.1016/0021-9673(91)85056-l.

DOI:10.1016/0021-9673(91)85056-l

PMID:1800524

Abstract

A method for the sensitive determination of the sulphopeptide lipoxins (LXs) C4, D4 and E4 by high-performance liquid chromatography with subsequent electrochemical detection is described. The best results were obtained when the analysis was carried out with the solvent system methanol-water-trifluoroacetic acid (66:34:0.008, v/v/v). The acquired half-wave potentials were different for all investigated compounds: +1.18 V for LXC4 +1.3 V for LXD4 and +1.25 V for LXE4. The detection limits of LXC4, LXD4 and LXE4, based on a signal-to-noise ratio of 3:1, were found to be 200-700 fmol. Although sulphopeptide lipoxins possess a high molar absorptivity, electrochemical detection still is three times more sensitive than ultraviolet detection.

摘要

描述了一种通过高效液相色谱法并随后进行电化学检测来灵敏测定硫肽脂氧素（LXs）C4、D4和E4的方法。当使用甲醇 - 水 - 三氟乙酸（66:34:0.008，v/v/v）的溶剂系统进行分析时，可获得最佳结果。所有研究化合物的半波电位均不同：LXC4为 +1.18 V，LXD4为 +1.3 V，LXE4为 +1.25 V。基于3:1的信噪比，LXC4、LXD4和LXE4的检测限为200 - 700 fmol。尽管硫肽脂氧素具有高摩尔吸光系数，但电化学检测仍比紫外检测灵敏三倍。