Nakajima N, Tanaka T, Miyachi Y, Imamura S, Kakizuka A
Department of Dermatology, Faculty of Medicine, Kyoto University, Japan.
J Dermatol. 1991 Nov;18(11):627-34. doi: 10.1111/j.1346-8138.1991.tb03146.x.
Various samples from lymphoproliferative diseases in the skin were analyzed by Southern blotting technique with probes from the T cell receptor gene, immunoglobulin genes, and human T cell leukemia virus-I genome. Samples were taken from 10 mycosis fungoides (MF) patients, 1 parapsoriasis en plaque patient, 10 Adult T cell leukemia/lymphoma (ATL) patients, 1 cutaneous T cell lymphoma (CTCL) patient, 4 lymphomatoid papulosis (LP) patients, 4 B cell lymphoma patients, and 2 actinic reticuloid (AR) patients. In MF, the monoclonality of the T cells became detectable first in the skin when plaques develop to tumors then in lymph nodes, and finally in the blood lymphocytes, indicating this disease develops from local (skin) malignancy to systemic malignancy. In parapsoriasis en plaque, no monoclonality was detected in any sample. We could distinguish cutaneous ATL from the carrier state by detecting the T cell monoclonality and HTLV-I integration with these probes. One patient with CTCL showed detectable T cell monoclonality; 1 out of 4 patients with LP did the same. Four samples from patients with B cell lymphoma revealed detectable monoclonal rearrangement of immunoglobulin heavy and light chain genes. In AR, no monoclonality was detected in any sample. From these data, we conclude that DNA analysis is useful in determining the monoclonality, cell origin, and distribution of monoclonal cells from skin samples.
采用T细胞受体基因、免疫球蛋白基因及人类T细胞白血病病毒I型基因组探针，通过Southern印迹技术对皮肤淋巴增生性疾病的各种样本进行了分析。样本取自10例蕈样肉芽肿（MF）患者、1例斑块状副银屑病患者、10例成人T细胞白血病/淋巴瘤（ATL）患者、1例皮肤T细胞淋巴瘤（CTCL）患者、4例淋巴瘤样丘疹病（LP）患者、4例B细胞淋巴瘤患者及2例光化性类网状细胞增生症（AR）患者。在MF中，当斑块发展为肿瘤时，首先在皮肤中可检测到T细胞的单克隆性，然后在淋巴结中，最终在血液淋巴细胞中检测到，这表明该疾病从局部（皮肤）恶性肿瘤发展为系统性恶性肿瘤。在斑块状副银屑病中，任何样本均未检测到单克隆性。通过检测T细胞单克隆性及HTLV-I整合情况，我们能够将皮肤ATL与携带状态区分开来。1例CTCL患者显示可检测到T细胞单克隆性；4例LP患者中有1例也是如此。4例B细胞淋巴瘤患者的样本显示免疫球蛋白重链和轻链基因存在可检测到的单克隆重排。在AR中，任何样本均未检测到单克隆性。根据这些数据，我们得出结论，DNA分析有助于确定皮肤样本中细胞的单克隆性、细胞起源及单克隆细胞的分布。
