一种用于双链RNA病毒的反向遗传学系统。

A Reverse genetics system for dsRNA viruses.

作者信息

Baric Ralph S, Sims Amy C

机构信息

Department of Epidemiology, School of Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

出版信息

Cell Host Microbe. 2007 Apr 19;1(2):90-1. doi: 10.1016/j.chom.2007.04.004.

DOI:10.1016/j.chom.2007.04.004

PMID:18005686

Abstract

The advent of a new reverse genetics system for reoviruses, described in this issue of Cell Host & Microbe by Kobayashi et al., opens new horizons of investigation into the molecular mechanisms governing dsRNA virus replication and pathogenesis. The elegant approach for recombinant reovirus recovery from a set of plasmid DNAs provides a template for designing similar reverse genetics systems for other dsRNA viruses associated with considerable morbidity and mortality in humans.

摘要

小林等人在本期《细胞宿主与微生物》中描述的呼肠孤病毒新型反向遗传学系统的出现，为研究双链RNA病毒复制和发病机制的分子机制开辟了新的视野。从一组质粒DNA中回收重组呼肠孤病毒的巧妙方法，为设计针对其他与人类相当高发病率和死亡率相关的双链RNA病毒的类似反向遗传学系统提供了模板。