Martins Liliane Ferreira, Yegles Michel, Thieme Detlef, Wennig Robert
National Laboratory of Health, Toxicology Division, University of Luxembourg, 162A avenue de la Faïencerie, L-1511, Luxembourg.
Forensic Sci Int. 2008 Mar 21;176(1):38-41. doi: 10.1016/j.forsciint.2007.06.023. Epub 2007 Nov 14.
The aim of this work was to study the influence of hair bleaching on the enantiomeric ratios of amphetamine-type stimulants (ATS), including amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine and 3,4-methylenedioxyethylamphetamine. Hair specimens from 14 STA users were treated with a commercial bleaching product during 40 min. After alkaline digestion and solid-phase extraction of bleached and non-bleached hair, the STA enantiomers were derivatised with an in-house synthesised chiral reagent, the (2S,4R)-N-heptafluorobutyryl-4-heptafluorobutoyloxy-prolyl chloride. The diastereoisomers were quantified by GC/MS-NCI. The results showed that the concentrations of all enantiomers decreased in bleached hair in comparison with the non-treated hair (median values between 20 and 39%). The enantiomeric ratios of the STA in bleached hair were not significantly different from those determined in non-treated hair. Our findings pointed out that bleaching treatments decrease concentrations of STA in hair without influencing their enantiomeric ratios.
这项工作的目的是研究头发漂白对苯丙胺类兴奋剂（ATS）对映体比例的影响，其中包括苯丙胺、甲基苯丙胺、3,4-亚甲基二氧基苯丙胺、3,4-亚甲基二氧基甲基苯丙胺和3,4-亚甲基二氧基乙基苯丙胺。14名使用苯丙胺类兴奋剂的用户的头发样本用一种市售漂白产品处理40分钟。对漂白和未漂白的头发进行碱性消化和固相萃取后，用自行合成的手性试剂（2S,4R）-N-七氟丁酰基-4-七氟丁酰氧基-脯氨酰氯对苯丙胺类兴奋剂对映体进行衍生化。通过气相色谱/质谱-负离子化学电离法对非对映异构体进行定量分析。结果表明，与未处理的头发相比，漂白头发中所有对映体的浓度均降低（中位数在20%至39%之间）。漂白头发中苯丙胺类兴奋剂的对映体比例与未处理头发中测定的比例无显著差异。我们的研究结果指出，漂白处理会降低头发中苯丙胺类兴奋剂的浓度，但不会影响其对映体比例。
