Cavaleiro A J, Pereira M A, Alves M
IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Bioresour Technol. 2008 Jul;99(10):4086-95. doi: 10.1016/j.biortech.2007.09.005. Epub 2007 Nov 19.
Two anaerobic sludges previously loaded with oleate and palmitate accumulated 4570+/-257 and 5200+/-9 mgCOD-LCFAgVSS(-1), respectively. These sludges were incubated in batch assays and methane production was recorded after addition of 100-900 mg L(-1) of oleate and palmitate, respectively. The batch assays were conducted before and after allowing the depletion of the biomass-associated LCFA. The presence of biomass-associated LCFA decreased the capacity of both sludges to convert the added LCFA to methane. After the degradation of biomass-associated LCFA, the lag phases observed before the onset of methane production were significantly reduced, evidencing an increase in the tolerance of the acetotrophic methanogens to the presence of LCFA. In another experiment, three recurrent pulses were performed with a real dairy wastewater containing 3.6 gCOD L(-1), from which 53% was fat. Methane yields of 0.45+/-0.01, 0.88+/-0.02 and 1.29+/-0.08 gCOD-CH(4) gCOD(fed)(-1) were achieved in the first, second and third pulses, respectively, evidencing an increasing capacity of the sludge to convert substrate accumulated in previous additions.
先前分别加载了油酸酯和棕榈酸酯的两种厌氧污泥，其积累的长链脂肪酸（LCFA）分别为4570±257和5200±9 mgCOD-LCFA/gVSS（-1）。将这些污泥进行分批试验，分别添加100 - 900 mg L（-1）的油酸酯和棕榈酸酯后记录甲烷产量。分批试验在生物质相关的LCFA耗尽前后进行。生物质相关LCFA的存在降低了两种污泥将添加的LCFA转化为甲烷的能力。在生物质相关LCFA降解后，甲烷产生开始前观察到的滞后期显著缩短，这表明乙酸营养型产甲烷菌对LCFA存在的耐受性增强。在另一项实验中，对含有3.6 gCOD L（-1）（其中53%为脂肪）的实际乳品废水进行了三次重复脉冲实验。在第一次、第二次和第三次脉冲实验中，甲烷产量分别达到0.45±0.01、0.88±0.02和1.29±0.08 gCOD-CH₄/gCOD（进料）（-1），这表明污泥将先前添加中积累的底物转化的能力不断增强。
