Dierick Herman A
The Neurosciences Institute, 10640 John J. Hopkins Drive, San Diego, California 92121, USA.
Nat Protoc. 2007;2(11):2712-8. doi: 10.1038/nprot.2007.404.
Aggressive behavior is a complex social behavior that is difficult to measure. Here, we describe a simple method for the quantitative analysis of aggression in male Drosophila melanogaster. Traditional measurements of aggressive behavior have relied on a territorial context with a food territory and a female as factors that induce or enhance aggression. The protocol described here is devoid of a food territory or a female, making it simpler than most existing methods used to measure aggressive behavior. Multiple pairs of males are tested simultaneously to obtain an average fighting score. Four parameters are used to quantify the behavior: frequency, index, latency and intensity of fighting based on unambiguous offensive fighting behaviors. The assay takes 15 min, during which time a frequency score is obtained for 20-35 pairs simultaneously. More in-depth analysis, including latency, index and intensity, can be performed on the videotaped record of the experiment. The assay is highly reproducible and requires limited resources in a simple setup.
攻击行为是一种复杂的社会行为，难以测量。在此，我们描述一种用于定量分析雄性黑腹果蝇攻击行为的简单方法。传统的攻击行为测量依赖于以食物领地和雌性作为诱导或增强攻击行为因素的领地情境。此处描述的实验方案没有食物领地或雌性，使其比大多数现有用于测量攻击行为的方法更简单。同时测试多对雄性果蝇以获得平均战斗得分。使用四个参数来量化该行为：基于明确的攻击性战斗行为的战斗频率、指数、潜伏期和强度。该测定需要15分钟，在此期间可同时获得20 - 35对果蝇的频率得分。可以对实验的录像记录进行更深入的分析，包括潜伏期、指数和强度。该测定具有高度可重复性，并且在简单设置下所需资源有限。
