采用响应面法优化玻璃微电极性能

Optimization of glass microelectrode properties by response surface methodology.

作者信息

Kisaalita W S, Skeen R S, Van Wie B J, Barnes C D, Fung S J

机构信息

Department of Chemical Engineering, Washington State University, Pullman 99164.

出版信息

J Neurosci Methods. 1991 Dec;40(2-3):113-20. doi: 10.1016/0165-0270(91)90059-9.

DOI:10.1016/0165-0270(91)90059-9

PMID:1800847

Abstract

Glass microelectrodes filled with electrolyte solutions are standard tools for electrophysiological studies. However, for any given application, there are limitations to the properties of the microelectrode, such as impedance and shank length, that can yield satisfactory results. The trial and error approach in pulling electrodes with the desired properties can be time consuming. The use of a response surface procedure which allows the experimenter to change more than one factor at a time and therefore determine the desired puller condition more efficiently is demonstrated. Also, design improvements for the World Precision Instrument, Model PUL-1, Microelectrode puller, used in this study are suggested.

摘要

填充电解质溶液的玻璃微电极是电生理研究的标准工具。然而，对于任何给定的应用，微电极的特性（如阻抗和柄长）存在局限性，这些局限性可能会产生令人满意的结果。通过反复试验的方法拉制具有所需特性的电极可能会很耗时。本文展示了使用响应面程序的方法，该方法允许实验者一次改变多个因素，从而更有效地确定所需的拉制条件。此外，还针对本研究中使用的世界精密仪器公司的PUL-1型微电极拉制器提出了设计改进建议。