Das S R, Baer H P
Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
Trop Med Parasitol. 1991 Sep;42(3):161-3.
The effects of the nucleoside analogs tubercidin, nebularin, formycin B and 3'-deoxyinosine on axenically grown Entamoeba histolytica were tested. Both tubercidin and nebularin showed pronounced inhibitory action, 50% of growth inhibition (IC50) being obtained at 0.14 and 0.82 microM, respectively. Formycin B and 3'-deoxyinosine were essentially inactive or weakly active at concentrations above 10 microM. Natural nucleosides, including adenosine, inosine, guanosine, thymidine, uridine and cytidine caused no significant effects at concentrations of 0.01-1 microM, however, significant inhibitory action was observed at or above 10 microM with cytidine and thymidine. The exploration of cytotoxic nucleosides as antiamebal drugs is of continued interest.
测试了核苷类似物杀结核菌素、喷司他丁、间型霉素B和3'-脱氧肌苷对无菌培养的溶组织内阿米巴的作用。杀结核菌素和喷司他丁均显示出明显的抑制作用，分别在0.14和0.82微摩尔浓度时获得50%的生长抑制（IC50）。间型霉素B和3'-脱氧肌苷在浓度高于10微摩尔时基本无活性或活性较弱。天然核苷，包括腺苷、肌苷、鸟苷、胸腺嘧啶核苷、尿苷和胞苷，在0.01 - 1微摩尔浓度时无显著影响，然而，胞苷和胸腺嘧啶核苷在10微摩尔及以上浓度时观察到显著的抑制作用。探索细胞毒性核苷作为抗阿米巴药物一直备受关注。
