Manandhar G, Khodiakov A L, Onishchenko G E
Tsitologiia. 1991;33(3):39-47.
A new method of cell fusion is proposed utilizing treatment with 15% solution of DMSO in serum before and after PEG treatment. With such treatments in SPEV cell culture a higher rate of cell fusion was obtained than that with other known methods of cell fusion. In the first wave of mitoses (0.5-4 h) mainly asynchronous division of nuclei, premature chromosome condensation and formation of telophase-like nuclei were observed in polykaryons. In the period of the second wave (14-20 h), mitoses were mainly synchronous and completed with cytokinesis. Micronuclei were formed frequently as a result of such mitoses. After the first wave of mitoses the number of polykaryons with pycnotic chromosomes sharply increased, and after the second wave of mitoses the number of polykaryons with pycnotic nuclei increased. The results obtained allow to conclude that heterophasic condition of the fused cells is one of the causes of pathological mitosis of polykaryons and of their death.
提出了一种新的细胞融合方法,即在聚乙二醇(PEG)处理前后,用含15%二甲基亚砜(DMSO)的血清溶液进行处理。通过在SPEV细胞培养中采用这种处理方法,获得了比其他已知细胞融合方法更高的细胞融合率。在第一轮有丝分裂(0.5 - 4小时)中,多核体中主要观察到细胞核的异步分裂、染色体过早凝聚以及类末期细胞核的形成。在第二轮(14 - 20小时)期间,有丝分裂主要是同步的,并伴有胞质分裂完成。由于这种有丝分裂,经常会形成微核。在第一轮有丝分裂后,具有固缩染色体的多核体数量急剧增加,而在第二轮有丝分裂后,具有固缩细胞核的多核体数量增加。所获得的结果表明,融合细胞的异相状态是多核体病理性有丝分裂及其死亡的原因之一。