Onishchenko G E, Petrashchuk O M
Tsitologiia. 1994;36(12):1187-91.
Localization of vimentin filaments has been investigated in polykaryons formed by cell fusion in culture of Chinese hamster cells (line Ag17). A network of vimentin filaments was observed around each nucleus of the interphase polykaryons. This compartmentalization of the network persists after beginning of mitoses up to metaphase, both in bipolar and multipolar mitotic cells. The picture of localization of intermediate filaments is most clear in polykaryons with mitotic asynchrony of the nuclei at early stages of mitosis. In anaphase, the central part of the network disappears, and in the polykaryon only peripheral part of the network is observed. The compartmentalization of the network of intermediate filaments is disrupted by such a way. In telophase, bright peripheral fluorescence may by the result of the beginning of network reorganization, and this process will be finished in daughter cells. The role of intermediate filaments in distribution of chromosomes and centrioles between daughter cells during mitosis is discussed.
在体外培养的中国仓鼠细胞（Ag17系）通过细胞融合形成的多核体中，对波形蛋白丝的定位进行了研究。在间期多核体的每个细胞核周围观察到波形蛋白丝网络。在有丝分裂开始直至中期，无论是双极还是多极有丝分裂细胞中，该网络的这种区室化都持续存在。在有丝分裂早期细胞核有丝分裂不同步的多核体中，中间丝的定位情况最为清晰。在后期，网络的中央部分消失，在多核体中仅观察到网络的外围部分。中间丝网络的这种区室化以这种方式被破坏。在末期，明亮的外围荧光可能是网络重组开始的结果，并且这个过程将在子细胞中完成。文中讨论了中间丝在有丝分裂期间染色体和中心粒在子细胞间分配中的作用。
