Flügel C, Lütjen-Drecoll E, Zadunaisky J A
Institute of Anatomy, University of Erlangen-Nürnberg, FRG.
Acta Histochem. 1991;91(1):67-75. doi: 10.1016/S0065-1281(11)80297-7.
Gills and operculum of seawater- and freshwater-adapted killyfish (Fundulus heteroclitus) were stained histochemically for carbonic anhydrase (CA). In the seawater-acclimatized specimens, CA was found predominantly in the chloride cells which were considerably larger than in the freshwater-adapted ones. Within these cells, the reaction products were concentrated in the apical parts of the cytoplasm. In contrast, chloride cells of freshwater-adapted fish were not, or only faintly, stained both in gills and opercular epithelium. Reaction products for CA were seen additionally in the cytoplasm of the outer respiratory cells lining the lamellae of gills both in seawater- and freshwater-adapted fish.
对适应海水和淡水的鳉鱼（Fundulus heteroclitus）的鳃和鳃盖进行了碳酸酐酶（CA）的组织化学染色。在适应海水环境的标本中，CA主要存在于氯化物细胞中，这些细胞比适应淡水环境的标本中的氯化物细胞大得多。在这些细胞内，反应产物集中在细胞质的顶端部分。相比之下，适应淡水环境的鱼的氯化物细胞在鳃和鳃盖上皮中未被染色或仅被微弱染色。在适应海水和淡水环境的鱼的鳃小片内衬的外呼吸细胞的细胞质中也额外观察到了CA的反应产物。
