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[一种用于测定毛细血管血中淋巴细胞亚群的全血微量方法,与其他间接免疫荧光方法的比较]

[A whole-blood micromethod to determine lymphocyte subsets in capillary blood, comparison with other methods of indirect immunofluorescence].

作者信息

Schäffner G, Seeger E, Volk H D, Fischer R, Kiowski S

机构信息

Staatliches Kontrollinstitut für immunbiologische Arzneimittel, Humboldt-Universität zu Berlin.

出版信息

Allerg Immunol (Leipz). 1991;37(2):103-8.

PMID:1801592
Abstract

Six lymphocyte antigens were detected with monoclonal antibodies in 0.2 ml capillary blood by a whole blood micromethod. After the lysis of erythrocytes of one drop of blood the remaining cells were layered on slides and were stained by indirect immunofluorescence. The relative and absolute counts of the lymphocyte subsets were of normal value and did not differ from the counts of flow cytometry and of slide and tube tests.

摘要

采用全血微量法,用单克隆抗体在0.2ml毛细血管血中检测到六种淋巴细胞抗原。一滴血液的红细胞裂解后,将剩余细胞铺在载玻片上,通过间接免疫荧光染色。淋巴细胞亚群的相对计数和绝对计数均为正常值,与流式细胞术以及玻片和试管检测的计数无差异。

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