Li R H, Zhuang L Z
Laboratory of Reproductive Biology, Chinese Academy of Sciences, Beijing, PRC.
Sci China B. 1991 Aug;34(8):938-46.
A new method of long-term culture of cytotrophoblast cells in serum-free medium has been developed. Cytotrophoblast cells were isolated with cold trypsin and purified by unit gravity sedimentation through BSA density gradients. The cells were cultured in the FD medium with supplement of EGF, insulin, transferrin and sodium selenite. They could survive over three weeks. The results showed that both EGF and insulin stimulated hCG and progesterone secretion and that sodium selenite elevated hCG output but not progesterone secretion. Transferrin produced synergistic effect with EGF and insulin on hCG and progesterone secretion but it was ineffective when used alone. This study demonstrates that the four growth factors mentioned above are essential for the survival of cytotrophoblast cells in vitro. It is therefore suggested that EGF, insulin and selenium may possibly be involved in the regulation of hCG and progesterone secretion in the human placenta.
一种在无血清培养基中长期培养细胞滋养层细胞的新方法已经建立。用冷胰蛋白酶分离细胞滋养层细胞，并通过BSA密度梯度单位重力沉降进行纯化。细胞在添加了表皮生长因子（EGF）、胰岛素、转铁蛋白和亚硒酸钠的FD培养基中培养。它们能够存活超过三周。结果表明，EGF和胰岛素均刺激人绒毛膜促性腺激素（hCG）和孕酮的分泌，而亚硒酸钠提高了hCG的产量，但不影响孕酮的分泌。转铁蛋白与EGF和胰岛素对hCG和孕酮的分泌产生协同作用，但单独使用时无效。本研究表明，上述四种生长因子对于体外细胞滋养层细胞的存活至关重要。因此，提示EGF、胰岛素和硒可能参与人胎盘hCG和孕酮分泌的调节。
