Kuang Yong, Huang Yue-Sheng
Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, The Third Military Medical University, Chongqing 400038, PR China.
Zhonghua Shao Shang Za Zhi. 2007 Jun;23(3):172-4.
To investigate the degree of injury to microtubule of myocardium at early post-hypoxia stage.
Cardiomyocytes from Wistar rats were isolated and cultured, and they were then divided into normal control and hypoxia groups. The distribution and morphological changes in microtubules were observed with laser confocal microscopy and scanning electron microscope at 10, 20, 30 post-hypoxia minutes (PHM) and 1 post-hypoxia hour (PHH). Then the fluorescence intensity of alpha-microtubule was detected with RT-PCR, the morphology of microtubule was observed, and the expression of dissociative alpha-microtubule was determined by Western blot.
Compared with normal control group, the bead-like structure of the microtubule in hypoxia group disappeared at 10 PHM, but no obvious change was observed in the distribution and number of microtubules. Despite the disappearance of bead-like structure of the microtubule, the microtubule derangement and loss of microtubule at the edge of cell were observed at 20 PHM. The fragmentation, derangement of texture, and loss of regularity in cardiomyocytes were observed at 30 PHM and 1 PHH. The fluorescence intensity of alpha-microtubule in hypoxia group was evidently decreased than that in normal group in a time-dependent manner. The expression of dissociative alpha-microtubule in hypoxia group at 10 PHM (46,644 +/- 145) was obviously higher than that in normal group (13,357 +/- 98, P < 0.01), and its increase was maintained with elapse of time.
Microtubule injury to cardiomyocytes occurs at early stage of post-hypoxia, with destruction of its structure and distribution.
探讨缺氧后早期心肌微管损伤程度。
分离培养Wistar大鼠心肌细胞，分为正常对照组和缺氧组。于缺氧后10、20、30分钟（PHM）及缺氧后1小时（PHH），用激光共聚焦显微镜和扫描电子显微镜观察微管的分布及形态变化。然后用RT-PCR检测α-微管的荧光强度，观察微管形态，并用蛋白质印迹法测定游离α-微管的表达。
与正常对照组相比，缺氧组微管的珠状结构在缺氧后10分钟消失，但微管的分布和数量无明显变化。尽管微管的珠状结构消失，但在缺氧后20分钟观察到微管排列紊乱及细胞边缘微管丢失。在缺氧后30分钟和1小时观察到心肌细胞破碎、纹理紊乱及规则性丧失。缺氧组α-微管的荧光强度明显低于正常组，且呈时间依赖性。缺氧组在缺氧后10分钟时游离α-微管的表达（46,644±145）明显高于正常组（13,357±98，P<0.01），且随时间延长其表达持续增加。
缺氧后早期心肌细胞微管发生损伤，其结构和分布遭到破坏。
