Hu Jiong-Yu, Huang Yue-Sheng, Song Hua-Pei, Zhang Dong-Xia, Xiang Fei, Zhu Zhi-Gang, Teng Miao, Zhang Qiong
Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing 400038, PR China.
Zhonghua Shao Shang Za Zhi. 2007 Jun;23(3):175-8.
To investigate and compare the protective effects of Astragaloside IV (AST) and Quercetin (QUE) on rat myocardial cells after their exposure to hypoxia, and to determine their dose-effect relationship.
Myocardial cells from fetal SD rat were cultured in vitro and divided into 7 groups: i.e. A (hypoxia), B (hypoxia and 100 mg/L of QUE), C (hypoxia and 50 mg/L of QUE), D (hypoxia and 25 mg/L of QUE), E (hypoxia and 50.0 mg/L of AST), F (hypoxia and 25.0 mg/L of AST), G (hypoxia and 12.5 mg/L AST) H(hypoxia and 10 mg/L of VitE) groups. Different doses of AST and QUE were added into the culture media cells in each group before the myocardial cells receiving hypoxia for 12 hrs. The number of viable cells (CCK-8) and the content of lactate dehydrogenase (LDH), superoxide dismutase (SOD), malondialdehyde (MDA), active oxygen (ROS, with detection only in A, C, F and H groups) were determined after hypoxia.
The amount of LDH, MDA, ROS (C, F groups) in group B - G decreased significantly compared with those of group A, while the number of viable cells and the SOD content increased significantly. The protective effects were better in group B - G than that of the group H. With the same dosage, levels of LDH, CCK-8 in AST-treated groups were significantly lower than those in QUE-treated group (the number of viable cells in group C, F was 0.454 +/- 0.018, 0.471 +/- 0.017, and the content of lactate dehydrogenase was 2800 +/- 9,2312 +/- 52). There were no significant differences in MDA, SOD and ROS levels between AST and QUE treated groups (ROS in C and F groups were 16.0 +/- 5.3 vs 22.4 +/- 8.7, P > 0.05).
AST and QUE might be beneficial in the protection of myocardial cells against hypoxia because of attenuation of oxidative damage. The protective effects of both AST and QUE are better than that of VitE, and that of AST is better than QUE as shown by a decrease in the amount of LDH and increase in the number of viable cells with the same dosage, but no obvious difference is shown between them in attenuating oxidative damage.
研究并比较黄芪甲苷（AST）和槲皮素（QUE）对缺氧大鼠心肌细胞的保护作用，并确定其剂量效应关系。
体外培养胎鼠SD大鼠心肌细胞，分为7组：即A组（缺氧组）、B组（缺氧+100mg/L QUE组）、C组（缺氧+50mg/L QUE组）、D组（缺氧+25mg/L QUE组）、E组（缺氧+50.0mg/L AST组）、F组（缺氧+25.0mg/L AST组）、G组（缺氧+12.5mg/L AST组）、H组（缺氧+10mg/L维生素E组）。在心肌细胞缺氧12小时前，向每组培养基中加入不同剂量的AST和QUE。缺氧后测定活细胞数量（CCK-8法）以及乳酸脱氢酶（LDH）、超氧化物歧化酶（SOD）、丙二醛（MDA）、活性氧（ROS，仅在A、C、F和H组检测）含量。
与A组相比，B - G组的LDH、MDA、ROS（C、F组）含量显著降低，而活细胞数量和SOD含量显著增加。B - G组的保护作用优于H组。相同剂量下，AST处理组的LDH、CCK-8水平显著低于QUE处理组（C、F组活细胞数量分别为0.454±0.018、0.471±0.017，乳酸脱氢酶含量分别为2800±9、2312±52）。AST和QUE处理组之间的MDA、SOD和ROS水平无显著差异（C、F组ROS分别为16.0±5.3和22.4±8.7，P>0.05）。
AST和QUE可能通过减轻氧化损伤对心肌细胞缺氧起到保护作用。AST和QUE的保护作用均优于维生素E，且相同剂量下，AST在降低LDH含量和增加活细胞数量方面的保护作用优于QUE，但在减轻氧化损伤方面两者无明显差异。
