Tsai Yu-Chen, Huang Jing-Dae
Department of Chemical Engineering, National Chung Hsing University, Taichung 402, Taiwan, ROC.
J Nanosci Nanotechnol. 2007 Sep;7(9):3227-32. doi: 10.1166/jnn.2007.691.
Novel nanobiocomposites multiwalled carbon nanotube-poly(vinyl alcohol)-glucose oxidase have been successfully prepared by a simple solution-evaporation method. The morphology and performance of the multiwalled carbon nanotube-poly(vinyl alcohol)-glucose oxidase film have been characterized by atomic force microscopy, cyclic voltammetry, and amperometry. The multiwalled carbon nanotube and glucose oxidase were observed to be homogeneously dispersed throughout the poly(vinyl alcohol) matrix. When compared with bare glassy carbon electrode, the sensitivity to hydrogen peroxide is greatly improved by about 115 times at multiwalled carbon nanotubepoly(vinyl alcohol) modified glassy carbon electrode. The glucose biosensor sensitivity was strongly influenced by the glucose oxidase concentration within the multiwalled carbon nanotube-poly(vinyl alcohol)-glucose oxidase composite.
通过一种简单的溶液蒸发法成功制备了新型纳米生物复合材料——多壁碳纳米管-聚乙烯醇-葡萄糖氧化酶。采用原子力显微镜、循环伏安法和安培法对多壁碳纳米管-聚乙烯醇-葡萄糖氧化酶膜的形态和性能进行了表征。观察到多壁碳纳米管和葡萄糖氧化酶均匀分散在整个聚乙烯醇基质中。与裸玻碳电极相比，多壁碳纳米管-聚乙烯醇修饰的玻碳电极对过氧化氢的灵敏度提高了约115倍。多壁碳纳米管-聚乙烯醇-葡萄糖氧化酶复合材料中葡萄糖氧化酶的浓度对葡萄糖生物传感器的灵敏度有很大影响。
