Rakhi Raghavan Baby, Sethupathi Kanikrishnan, Ramaprabhu Sundara
Alternative Energy and Nanotechnology Laboratory, Nano Functional Materials Technology Centre (NFMTC), Department of Physics, Indian Institute of Technology Madras, Chennai-600036.
J Phys Chem B. 2009 Mar 12;113(10):3190-4. doi: 10.1021/jp810235v.
A new amperometric biosensor, based on deposition of glucose oxidase (GOD) onto crystalline gold (Au) nanoparticle modified multiwalled carbon nanotube (MWNT) electrode, is presented. MWNTs have been synthesized by catalytic chemical vapor decomposition of acetylene over rare-earth-based AB2 (DyNi2) alloy hydride catalyst. Purified MWNTs have been decorated with nanocrystalline Au metal clusters using a simple chemical reduction method. The characterization of metal-decorated CNTs has been done using X-ray diffraction analysis, scanning electron microscopy, transmission electron microscopy (TEM), high-resolution TEM, and energy-dispersive X-ray analysis. Amperometric biosensor fabricated by depositing GOD over Nafion-solubilized Au-MWNT electrode retains its biocatalytic activity and offers fast and sensitive glucose quantification. The performance of the biosensor has been studied using cyclic voltammetry, amperometry, and hydrodynamic voltammetry, and the results have been discussed. The fabricated glucose biosensor exhibits a linear response up to 22 mM glucose and a detection limit of 20 microM.
本文介绍了一种新型安培生物传感器，它是基于将葡萄糖氧化酶（GOD）沉积在结晶金（Au）纳米颗粒修饰的多壁碳纳米管（MWNT）电极上构建而成。MWNTs通过乙炔在稀土基AB2（DyNi2）合金氢化物催化剂上的催化化学气相分解法合成。采用简单的化学还原法，用纳米晶Au金属簇修饰纯化后的MWNTs。通过X射线衍射分析、扫描电子显微镜、透射电子显微镜（TEM）、高分辨率TEM和能量色散X射线分析对金属修饰的碳纳米管进行了表征。通过在Nafion溶解的Au-MWNT电极上沉积GOD制备的安培生物传感器保留了其生物催化活性，并能实现快速、灵敏的葡萄糖定量分析。利用循环伏安法、安培法和流体动力学伏安法研究了该生物传感器的性能，并对结果进行了讨论。所制备的葡萄糖生物传感器在高达22 mM葡萄糖浓度范围内呈现线性响应，检测限为20 microM。
