采用落射荧光显微镜评估噬菌体衣壳的完整性。

Epifluorescent microscopy to evaluate bacteriophage capsid integrity.

作者信息

Naicker Kerusha, Durbach Steven I

机构信息

University of the Witwatersrand, Johannesburg, Gauteng, South Africa.

出版信息

Biotechniques. 2007 Oct;43(4):473-4, 476. doi: 10.2144/000112584.

DOI:10.2144/000112584

PMID:18019338

Abstract

The standard method to evaluate capsid integrity of DNA-based viruses, which depends on access to DNase I, relies on the removal of the capsid by solvent extraction and then the evaluation of the nucleic acid products by electrophoresis. Our method, which is based on the direct detection of capsid-borne DNA directly through the use of epifluorescent microscopy, negates the requirement of DNA extraction.

摘要

评估基于DNA的病毒衣壳完整性的标准方法依赖于使用DNase I，该方法通过溶剂萃取去除衣壳，然后通过电泳评估核酸产物。我们的方法基于使用落射荧光显微镜直接检测衣壳携带的DNA，无需进行DNA提取。