用于人类身份鉴定的短串联重复序列分型技术。
Short tandem repeat typing technologies used in human identity testing.
作者信息
Butler John M
机构信息
Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD 20899-8311, USA.
出版信息
Biotechniques. 2007 Oct;43(4):ii-v. doi: 10.2144/000112582.
Short tandem repeat (STR) typing methods are widely used today for human identity testing applications including forensic DNA analysis. Following multiplex PCR amplification, DNA samples containing the length-variant STR alleles are typically separated by capillary electrophoresis and genotyped by comparison to an allelic ladder supplied with a commercial kit. This article offers a brief perspective on the technologies and issues involved in STR typing.
短串联重复序列（STR）分型方法如今广泛应用于人类身份鉴定领域，包括法医DNA分析。在多重PCR扩增之后，含有长度可变的STR等位基因的DNA样本通常通过毛细管电泳进行分离，并通过与商业试剂盒提供的等位基因分型标准物进行比较来进行基因分型。本文简要介绍了STR分型所涉及的技术和问题。
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