Baron P L, Meola G, Scarpini E, Moggio M, Santilli I, Scarlato G
Istituto di Clinica Neurologica, Centro Dino Ferrari, Università degli Milano, Italy.
Eur J Basic Appl Histochem. 1991;35(4):423-32.
We studied the distribution of lysosomes in differentiating human muscle cells in culture treated with propranolol, leupeptin and chloroquine. Chloroquine treated cells showed a significant vacuolization and an increase of the lysosomal apparatus as assessed by cytochemical analysis of the lysosomal enzyme acid phosphatase and by acridine orange staining. At electron microscopic level, an increase of lysosome-like bodies and disorganization of the contractile apparatus were demonstrated in multinucleated myotubes. These alterations observed in cultured muscle cells suggest that lysosomotropic agents may be harmful.
我们研究了用普萘洛尔、亮肽素和氯喹处理的培养中分化的人肌肉细胞中溶酶体的分布。通过溶酶体酶酸性磷酸酶的细胞化学分析和吖啶橙染色评估，氯喹处理的细胞显示出明显的空泡化和溶酶体装置增加。在电子显微镜水平上，多核肌管中显示出类溶酶体小体增加和收缩装置紊乱。在培养的肌肉细胞中观察到的这些改变表明溶酶体促效剂可能是有害的。
