Clarke David J, Campopiano Dominic J
School of Chemistry, EaStChem, University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, UK.
Org Biomol Chem. 2007 Aug 21;5(16):2564-6. doi: 10.1039/b708478a.
Microcin J25 is a ribosomally synthesised 21-residue antimicrobial peptide produced by certain strains of enterobacteria, that adopts an extraordinary 'threaded lasso' structure. To date, the biosynthesis of this peptide is little understood. Here we report the in vitro maturation of the microcin precursor peptide into active microcin J25 for the first time. Furthermore, we show that the enzymes required for the posttranslational modification of this precursor peptide are associated with the bacterial inner membrane.
微菌素J25是一种由某些肠杆菌菌株产生的核糖体合成的21个氨基酸残基的抗菌肽，它具有一种独特的“穿线套索”结构。迄今为止，这种肽的生物合成还鲜为人知。在此，我们首次报道了微菌素前体肽在体外成熟为活性微菌素J25的过程。此外，我们还表明，这种前体肽进行翻译后修饰所需的酶与细菌内膜相关。
