Schmidt W, Sandor S, Checiu I, Stefănescu S, Havlik I, Sere I, Checiu M, Fazakas-Todea I, Eremia I
Center of Chemistry, Timişoara, Romania.
Rom J Morphol Embryol. 1991 Jan-Jun;37(1-2):31-9.
Since 1948 trypan blue has been a well-known and extensively used experimental teratogen, belonging to the group of azo dyes. Chemically, trypan blue consists of a biphenyl molecule (0-tolidine or benzidine) combined by means of azo linkages with two molecules of a substituted naphthalene. Between 1987-89 the effect of the replacement of the biphenyl molecule by a molecule of p,p'-diaminobenzanilide upon the prenatal noxious action of trypan blue has been controlled. Investigations were carried out on three species: chick embryos, albino rats and albino mice. In the species used, the replacement annihilates the teratogenic properties of the dye, with the persistence of some embryotoxic effects. On the other hand, the control of o-tolidine and of p,p'-diaminobenzanilide revealed that no one had teratogenic properties (only some embryotoxic effect, more marked in the case of o-tolidine). It results that the teratogenic action of trypan blue cannot be attributed to the o-tolidine molecule proper but to an effect which results (in a for the moment unknown manner) from its combination with the other parts of the dye molecule.
自1948年以来，锥虫蓝一直是一种广为人知且被广泛使用的实验性致畸剂，属于偶氮染料类。从化学结构上看，锥虫蓝由一个联苯分子（邻联甲苯胺或联苯胺）通过偶氮键与两个取代萘分子结合而成。在1987年至1989年期间，研究了用对，对'-二氨基苯甲酰替苯胺分子取代联苯分子后对锥虫蓝产前有害作用的影响。对三种物种进行了研究：鸡胚、白化大鼠和白化小鼠。在所使用的物种中，这种取代消除了染料的致畸特性，但仍存在一些胚胎毒性作用。另一方面，对联甲苯胺和对，对'-二氨基苯甲酰替苯胺的研究表明，它们都没有致畸特性（只有一些胚胎毒性作用，邻联甲苯胺的情况更明显）。结果表明，锥虫蓝的致畸作用不能归因于邻联甲苯胺分子本身，而是归因于它与染料分子其他部分结合产生的一种效应（目前其方式尚不清楚）。
