Kinasiewicz A, Dudziński K, Chwojnowski A, Weryński A, Kawiak J
Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Warsaw, Poland.
Transplant Proc. 2007 Nov;39(9):2914-6. doi: 10.1016/j.transproceed.2007.08.061.
Implantable bioartificial liver has been investigated for patients suffering from liver insufficiency after mass liver resection or acute liver failure. Liver cells are implanted as free cell suspension, in microencapsulation systems or using microcarriers. To exhibit their typical functions, hepatic cells need a three-dimensional environment that is much more physiological than a flat one. The aim of our study was in vivo evaluation of spongy polyethersulfone membranes as a synthetic support for hepatic cells grown three dimensionally and transplanted to SCID/NOD mice. Spongy membranes were prepared using phase inversion from membrane-forming mixtures containing the following: polyethersulfone (based polymer), dimethylformamide (solvent), polyvinylpyrrolidone MW 10000 (small pore precursor), and cellulose (large pore precursor). We observed that polyethersulfone membranes were well tolerated by C3A cells and we did not observe any toxic effect, resulting in viability of cells >95%. Use of collagen gel as a support for cells on the scaffold gives the opportunity to increase 10 times the number of cells seeded on the membrane. Heparin addition to collagen gel did not influence albumin production in SCID/NOD mice. We observed an increase of albumin production after 7 and 14 days after implantation. Use of collagen gels in combination with polymer scaffolds allows preparation of bioartificial organs possessing high cell concentration for transplantation purposes.
对于在大规模肝切除术后或急性肝衰竭后出现肝功能不全的患者，人们已经对植入式生物人工肝进行了研究。肝细胞以游离细胞悬液的形式、在微囊化系统中或使用微载体进行植入。为了发挥其典型功能，肝细胞需要一个比平面环境更接近生理状态的三维环境。我们研究的目的是在体内评估海绵状聚醚砜膜作为三维生长并移植到SCID/NOD小鼠体内的肝细胞的合成支架。使用含有以下成分的成膜混合物通过相转化法制备海绵状膜：聚醚砜（基础聚合物）、二甲基甲酰胺（溶剂）、分子量为10000的聚乙烯吡咯烷酮（小孔前驱体）和纤维素（大孔前驱体）。我们观察到C3A细胞对聚醚砜膜耐受性良好，未观察到任何毒性作用，细胞活力>95%。使用胶原凝胶作为支架上细胞的支撑物，有机会使接种在膜上的细胞数量增加10倍。向胶原凝胶中添加肝素对SCID/NOD小鼠的白蛋白产生没有影响。我们观察到植入后7天和14天白蛋白产生增加。将胶原凝胶与聚合物支架结合使用，可以制备出具有高细胞浓度用于移植目的的生物人工器官。
