Kunzmann Andrea, Dedoussis George, Jajte Jolanta, Malavolta Marco, Mocchegiani Eugenio, Bürkle Alexander
Department of Biology, University of Konstanz, Konstanz, Germany.
Exp Gerontol. 2008 May;43(5):409-14. doi: 10.1016/j.exger.2007.10.003. Epub 2007 Oct 9.
Poly(ADP-ribosyl)ation is a posttranslational protein modification, which is catalyzed by poly(ADP-ribose) polymerase-1 (PARP-1) and plays a role in DNA repair and maintenance of genomic stability. A decrease in cellular poly(ADP-ribosyl)ation has been implicated in the aging process. As PARP-1 is a zinc finger protein its decreased function might be related to age-related zinc deficiency. To test this hypothesis we assessed cellular poly(ADP-ribosyl)ation capacity in 29 donors from Greece, Italy and Poland as function of age and nutritional zinc status. Our results reveal a positive correlation between cellular poly(ADP-ribosyl)ation capacity and zinc status in human peripheral blood mononuclear cells (PBMC) (p<0.05). We could also confirm a decrease of PARP-1 activity with donor age, highlighting the role of poly(ADP-ribosyl)ation in the aging process. The results demonstrate that zinc supplementation in elderly people can increase the cellular poly(ADP-ribosyl)ation capacity of their PBMC. We speculate that this may help maintain integrity and stability of the genome more efficiently and thus contribute to an extension of healthspan.
聚(ADP - 核糖)化是一种蛋白质翻译后修饰,由聚(ADP - 核糖)聚合酶 -1(PARP -1)催化,在DNA修复和基因组稳定性维持中发挥作用。细胞聚(ADP - 核糖)化的减少与衰老过程有关。由于PARP -1是一种锌指蛋白,其功能下降可能与年龄相关的锌缺乏有关。为了验证这一假设,我们评估了来自希腊、意大利和波兰的29名捐赠者的细胞聚(ADP - 核糖)化能力,作为年龄和营养锌状态的函数。我们的结果显示,人类外周血单核细胞(PBMC)中的细胞聚(ADP - 核糖)化能力与锌状态呈正相关(p<0.05)。我们还证实了PARP -1活性随捐赠者年龄的降低,突出了聚(ADP - 核糖)化在衰老过程中的作用。结果表明,老年人补充锌可以增加其PBMC的细胞聚(ADP - 核糖)化能力。我们推测,这可能有助于更有效地维持基因组的完整性和稳定性,从而有助于延长健康寿命。