[日本血吸虫抗独特型单克隆抗体NP30的建立与鉴定]
[The establishment and characterization of the anti-idiotypic monoclonal antibody-NP30 of Schistosoma japonicum].
作者信息
Guan X H, Qiu Z N, Ma L, Tao R H, Wu Y Q, He Q, Wu G L, Zhao W X
机构信息
Department of Parasitology, Nanjing Medical College.
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1991;9(4):261-4.
A hybridoma cell line secreting an IgM monoclonal antibody designated NP30 was obtained from a fusion of SP2/o and spleen cells of a BALB/c mouse chronically infected with schistosoma japonicum for one and a half year and identified by screening with immunized rabbit sera against gut-associated antigen (GAA) and soluble egg antigen (SEA) of S. japonicum, indicating that the NP30 was an anti-anti-antigen or anti-antibody. NP30 was further determined to be an anti-idiotypic antibody (anti-id) which was serologically and functionally identical to GAA, so that it could be portrayed as the internal image of GAA, which might have the potential to be used as an antigenic reagent in immunodiagnostic assays of schistosomiasis japonica.
通过将SP2/o细胞与一只慢性感染日本血吸虫半年的BALB/c小鼠的脾细胞进行融合，获得了一株分泌名为NP30的IgM单克隆抗体的杂交瘤细胞系，并通过用针对日本血吸虫肠相关抗原（GAA）和可溶性虫卵抗原（SEA）的免疫兔血清进行筛选鉴定，表明NP30是一种抗抗抗原或抗抗体。进一步确定NP30是一种独特型抗体（抗独特型），其在血清学和功能上与GAA相同，因此可以将其描绘为GAA的内影像，这可能有潜力用作日本血吸虫病免疫诊断检测中的抗原试剂。
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