Chen Ya-Feng, Shi Min, Huang Fang, Chen Xue-Xin
Institute of Insect Sciences, Zhejiang University, 268 Kaixuan Road, Hangzhou 310029, PR China.
J Gen Virol. 2007 Dec;88(Pt 12):3317-3322. doi: 10.1099/vir.0.82999-0.
Cotesia vestalis is an endoparasitoid of larval stages of Plutella xylostella, the diamondback moth. For successful parasitization, this parasitoid injects a polydnavirus into its host during oviposition. Here we isolated two genes, which we named CvBV1 and CvBV2. CvBV1 was located on segment CvBV-S5 with a size of 790 bp, while CvBV2 was located on segment CvBV-S51 with a size of 459 bp. A gene copy of CvBV2 was found on segment CvBV-S48, which we name CvBV2'. Gene duplication occurred in both genes, tandem gene duplication for CvBV1 and segmental duplication for CvBV2. Gene transcripts of the two genes were detected in hosts as early as 0.5 h post-parasitization (p.p.) and continued to be detected for six days, and tissue-specific expression patterns showed that they could be detected in the haemolymph and brain at 2 h p.p., suggesting that they could participate in early protection of parasitoid eggs from host cellular encapsulation.
菜蛾盘绒茧蜂是小菜蛾幼虫阶段的一种内寄生蜂。为了成功寄生，这种寄生蜂在产卵时会将一种多分DNA病毒注入其宿主。在此，我们分离出了两个基因，命名为CvBV1和CvBV2。CvBV1位于CvBV-S5片段上，大小为790 bp，而CvBV2位于CvBV-S51片段上，大小为459 bp。在CvBV-S48片段上发现了一个CvBV2基因拷贝，我们将其命名为CvBV2'。两个基因均发生了基因重复，CvBV1为串联基因重复，CvBV2为片段重复。早在寄生后0.5小时（p.p.）就在宿主中检测到了这两个基因的转录本，并且在六天内持续被检测到，组织特异性表达模式表明在寄生后2小时可以在血淋巴和大脑中检测到它们，这表明它们可能参与了寄生蜂卵早期免受宿主细胞包囊的保护。
