Ding Chuanqing, Rife Lawrence, Nakamura Tamako, Wang Yu-Wen, Kopp Kimberly, Schechter Joel E
Department of Cell and Neurobiology, University of Southern California, Los Angeles, CA 90089, USA.
Ophthalmic Res. 2008;40(1):49-52. doi: 10.1159/000111159. Epub 2007 Nov 20.
To develop a local approach to study rabbit lacrimal secretion in situ by administering specific secretagogues directly onto the lacrimal gland (LG).
After the rabbit has been anesthetized, the inferior bulbar conjunctiva and underlying connective tissue are blunt dissected. A polyethylene tube, for drug delivery, is inserted through the fibrous membrane overlying the inferior surface of the orbital cavity beneath which the LG resides. Lacrimal fluid is collected by cannulating the lacrimal duct.
Pilocarpine induced robust lacrimal fluid secretion from the LG that had been bathed with either pilocarpine or phenylephrine, with no detectable effect on the contralateral LG and salivary secretion. By next giving pilocarpine or phenylephrine to the control LG while the experimental gland used before served as control, we duplicated the results exactly.
This novel approach avoids the unwanted systemic effects of intravenous injection and is a promising technique to study rabbit LG secretion in situ.
开发一种局部给药方法，通过将特定促分泌剂直接应用于泪腺（LG）来原位研究兔泪液分泌。
兔麻醉后，钝性分离球结膜下及下方的结缔组织。将用于给药的聚乙烯管穿过覆盖眼眶下表面的纤维膜插入，泪腺位于该膜下方。通过泪道插管收集泪液。
毛果芸香碱可使已用毛果芸香碱或去氧肾上腺素处理过的泪腺产生大量泪液分泌，对侧泪腺和唾液分泌无明显影响。接下来，在将之前用作对照的实验腺体作为对照的同时，给对照泪腺给予毛果芸香碱或去氧肾上腺素，我们得到了完全相同的结果。
这种新方法避免了静脉注射带来的不必要的全身效应，是一种有前景的原位研究兔泪腺分泌的技术。
