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活性氧、精浆总抗氧化剂浓度及其对体外受精/卵胞浆内单精子注射患者精子参数和结局的影响

Reactive oxygen species, total antioxidant concentration of seminal plasma and their effect on sperm parameters and outcome of IVF/ICSI patients.

作者信息

Hammadeh M E, Al Hasani S, Rosenbaum P, Schmidt W, Fischer Hammadeh C

机构信息

Department of Obstetrics and Gynaecology, University of Saarlandes, 66421 Homburg/Saar, Germany.

出版信息

Arch Gynecol Obstet. 2008 Jun;277(6):515-26. doi: 10.1007/s00404-007-0507-1.

DOI:10.1007/s00404-007-0507-1

PMID:18026972

Abstract

OBJECTIVE

The purpose of this study was to determine and compare the concentration of reactive oxygen species (ROS) and total antioxident (TAS) in seminal plasma of IVF (in vitro fertilization) and ICSI patients, to establish their effect on sperm quality (count, vitality, HOS, morphology, maturity, DNA strand breaks) and assess the fertilization potential of spermatozoa and IVF/ICSI outcome.

METHOD

IVF/ICSI patients (n = 48) 26 IVF and 22 ICSI were included in this study. A spermiogram was generated from each patient one-hour post ejaculation and smears were made from each semen sample to evaluate the morphology, sperm maturity (Chromomycin CMA3) and DNA strand breaks (Terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labelling, TUNEL-assay).

RESULTS

In both groups a negative correlation was found between ROS concentration in seminal plasma and sperm vitality (r= -0.111; P = 0.453); membrane integrity and morphology (-0.141; P = 0.340) and fertilization rate (r = -0.0290; P=0.045). However, TAS in seminal plasma correlated positive with fertilization rate (r = 0.081; P = 0.584). In addition, an inverse correlation was found between sperm DNA strand breaks (TUNEL-test) and spermatozoa global and progressive motility, vitality, and membrane integrity. Furthermore, the mean percentage of normal condensed spermatozoa (CMA3) was significantly higher (P = 0.0001) in patients undergoing IVF compared to ICSI. Spermatozoa of male ICSI patients were more susceptible to acid denaturation (acridine orange staining) compared to spermatozoa of male IVF patients (P = 0.041). However, ROS concentration was higher in IVF patients compared to ICSI patients (94.73 +/- 102.84 vs. 54.78 +/- 39.83 micromol/l, whilst TAS levels (1.43 +/- 0.28 vs. 1.53 +/- 0.22) and fertilization rate (67. 26 vs. 67.26) were similar in both groups.

CONCLUSION

ROS concentration and other sperm parameters were higher in IVF compared to ICSI patients. TAS concentration was comparable between the two groups. However, the fertilization rate was smilar in IVF and ICSI patients. Therefore, ROS concentration in seminal plasma affects the quality of spermatozoa but does not affect the fertilization rate in IVF/ICSI cycles.

摘要

目的

本研究旨在测定并比较体外受精（IVF）和卵胞浆内单精子注射（ICSI）患者精液中活性氧（ROS）浓度和总抗氧化剂（TAS）水平，确定其对精子质量（数量、活力、低渗肿胀试验、形态、成熟度、DNA链断裂）的影响，并评估精子的受精潜能及IVF/ICSI结局。

方法

本研究纳入了48例IVF/ICSI患者（26例IVF患者和22例ICSI患者）。在射精后1小时为每位患者进行精液分析，并从每个精液样本制作涂片，以评估形态、精子成熟度（嗜铬霉素CMA3）和DNA链断裂情况（末端脱氧核苷酸转移酶介导的dUTP缺口末端标记，TUNEL检测）。

结果

在两组中，精液中ROS浓度与精子活力（r = -0.111；P = 0.453）、膜完整性和形态（r = -0.141；P = 0.340）以及受精率（r = -0.0290；P = 0.045）之间均呈负相关。然而，精液中TAS与受精率呈正相关（r = 0.081；P = 0.584）。此外，精子DNA链断裂（TUNEL检测）与精子整体及前向运动能力、活力和膜完整性之间呈负相关。此外，与ICSI患者相比，接受IVF的患者中正常浓缩精子（CMA3）的平均百分比显著更高（P = 0.0001）。与IVF男性患者的精子相比，ICSI男性患者的精子对酸变性（吖啶橙染色）更敏感（P = 0.041）。然而，与ICSI患者相比，IVF患者的ROS浓度更高（94.73±102.84对54.78±39.83微摩尔/升），而两组的TAS水平（1.43±0.28对1.53±0.22）和受精率（67.26对67.26）相似。