Mazina O M, Korochkina S E
Genetika. 1991 Nov;27(11):1920-7.
Using interstrain level ovary transplantations function of ovaries was estimated in females bearing mutations at the ecs locus mapped in the 2B3-5 region of the X-chromosome of Drosophila melanogaster. Dissection of recipient flies and genetic analysis of their progeny demonstrated that the donor ovary in fertile flies was capable of normal functioning in the case of connection of the donor ovary in fertile flies with the recipient oviducts. Therefore, female-sterile mutations at the ecs locus are somatic line specific. SDS-PAGE electrophoresis revealed normal level of the yolk proteins in such females. Thus, the sterility of the ecs mutants may arise from abnormal morphogenesis of their genital disc, due to the loss of normal sensitivity to ecdysterone.
利用品系间卵巢移植，对携带位于黑腹果蝇X染色体2B3 - 5区域ecs位点突变的雌性果蝇的卵巢功能进行了评估。对受体果蝇进行解剖并对其后代进行遗传分析，结果表明，在可育果蝇中，若将供体卵巢与受体输卵管相连，供体卵巢能够正常发挥功能。因此，ecs位点的雌性不育突变是体细胞系特异性的。SDS - PAGE电泳显示此类雌性果蝇中卵黄蛋白水平正常。因此，ecs突变体的不育可能是由于其生殖盘形态发生异常，这是由于对蜕皮激素失去了正常敏感性所致。
