Bode Ulrike, Lörchner Marc, Ahrendt Manuela, Blessenohl Maike, Kalies Kathrin, Claus Anja, Overbeck Silke, Rink Lothar, Pabst Reinhard
Functional and Applied Anatomy, Medical School Hannover, Hannover, Germany.
Immunology. 2008 Apr;123(4):480-90. doi: 10.1111/j.1365-2567.2007.02713.x. Epub 2007 Nov 20.
Dendritic cells (DC) are important in differential T-cell priming. Little is known about the local priming by DC in the microenvironment of different lymph nodes and about the fate of the imprinted T cells. Therefore, freshly isolated rat DC from mesenteric lymph nodes (mLN) and axillary lymph nodes (axLN) were phenotyped and cultured with blood T cells in the presence of the superantigen Mycoplasma arthritidis mitogen (MAM). The phenotype, proliferation and apoptosis of the primed T cells were analysed. Our data show that a common DC population exists in both mLN and axLN. In addition, region-specific DC with an organotypical marker expression imprinted by the drained area were found. Coculture of T cells with DC from mLN or axLN resulted in a distinct shift in the CD4 and CD8 expression of T cells and their phenotype. Furthermore, when these differentially primed mLN and axLN T cells were injected into recipients, mLN-primed T cells survived longer in other lymphoid organs. The results show that the region-specific DC have a unique phenotype and an impact on the ratio of CD4 : CD8 T cells during an immune response in vivo.
树突状细胞（DC）在T细胞的差异启动中起重要作用。关于DC在不同淋巴结微环境中的局部启动以及印记T细胞的命运，人们了解甚少。因此，对从肠系膜淋巴结（mLN）和腋窝淋巴结（axLN）新鲜分离的大鼠DC进行了表型分析，并在超抗原关节炎支原体有丝分裂原（MAM）存在的情况下与血液T细胞共培养。分析了启动后T细胞的表型、增殖和凋亡情况。我们的数据表明，mLN和axLN中存在共同的DC群体。此外，还发现了具有由引流区域印记的器官典型标志物表达的区域特异性DC。T细胞与来自mLN或axLN的DC共培养导致T细胞的CD4和CD8表达及其表型发生明显变化。此外，当将这些差异启动的mLN和axLN T细胞注射到受体中时，mLN启动的T细胞在其他淋巴器官中的存活时间更长。结果表明，区域特异性DC具有独特的表型，并且在体内免疫反应期间对CD4:CD8 T细胞的比例有影响。
