用于酿酒酵母中基于聚合酶链反应的基因破坏的改进方法。
Improved method for the PCR-based gene disruption in Saccharomyces cerevisiae.
作者信息
Koyama Hiroshi, Sumiya Eriko, Ito Takahiro, Sekimizu Kazuhisa
机构信息
Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, University of Tokyo, Bunkyo, Tokyo, Japan.
出版信息
FEMS Yeast Res. 2008 Mar;8(2):193-4. doi: 10.1111/j.1567-1364.2007.00334.x. Epub 2007 Nov 19.
The PCR-based gene disruption strategy originally devised by Baudin et al. is widely used for gene targeting in Saccharomyces cerevisiae. An advantage of this strategy is its simplicity in making targeting constructs. The efficiencies of the targeted disruption are highly variable from locus to locus, however, and often very low. In this report, a method for improving the gene deletion efficiency is described.
最初由鲍丁等人设计的基于聚合酶链反应（PCR）的基因破坏策略被广泛用于酿酒酵母中的基因靶向。该策略的一个优点是在构建靶向载体时操作简单。然而，靶向破坏的效率在不同基因座之间差异很大，而且通常很低。在本报告中，描述了一种提高基因缺失效率的方法。
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