• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Synthesis of homologous peptides using fragment condensation: analogs of an HIV proteinase substrate.

作者信息

Bláha I, Nemec J, Tözsér J, Oroszlan S

机构信息

Laboratory of Molecular Virology and Carcinogenesis, PRI/DynCorp., NCI-Frederick Cancer Research and Development Center, MD.

出版信息

Int J Pept Protein Res. 1991 Nov;38(5):453-8. doi: 10.1111/j.1399-3011.1991.tb01526.x.

DOI:10.1111/j.1399-3011.1991.tb01526.x
PMID:1802863
Abstract

Two protected peptides Boc-Val-Ser(Bzl)-Gln-Asn-Tyr(BrZ)OH and Boc-Val-Ser(Bzl)-Gln-Asn-Tyr(BrZ)-ProOH were synthesized on a resin substituted by 9-(hydroxymethyl)-2-fluoreneacetic acid. After cleavage with piperidine/DMF, desalting, and activation, these peptides were used for the synthesis of 11 analogs of an HIV proteinase nonapeptide substrate Val-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-NH2 using fragment condensation in solid phase. The fragment condensation was made in an ultrasonic bath. Using only 2 equivalents of the activated peptide in a DMF solution, this reaction was complete in 2 h. All nonapeptides were assayed as substrates for HIV-1 and HIV-2 proteinases.

摘要

相似文献

1
Synthesis of homologous peptides using fragment condensation: analogs of an HIV proteinase substrate.
Int J Pept Protein Res. 1991 Nov;38(5):453-8. doi: 10.1111/j.1399-3011.1991.tb01526.x.
2
Substitution of proline with pipecolic acid at the scissile bond converts a peptide substrate of HIV proteinase into a selective inhibitor.
Biochem Biophys Res Commun. 1990 May 31;169(1):310-4. doi: 10.1016/0006-291x(90)91469-9.
3
Studies on the role of the S4 substrate binding site of HIV proteinases.
FEBS Lett. 1991 Feb 25;279(2):356-60. doi: 10.1016/0014-5793(91)80186-7.
4
Kinetic and modeling studies of subsites S4-S3' of Moloney murine leukemia virus protease.莫洛尼鼠白血病病毒蛋白酶S4 - S3'亚位点的动力学与建模研究
J Biol Chem. 1994 Jun 17;269(24):16795-801.
5
Modified oligopeptides designed to interact with the HIV-1 proteinase inhibit viral replication.
Arch Virol. 1990;114(3-4):167-73. doi: 10.1007/BF01310746.
6
Human immunodeficiency virus-1 protease. 1. Initial velocity studies and kinetic characterization of reaction intermediates by 18O isotope exchange.
Biochemistry. 1991 Aug 27;30(34):8441-53. doi: 10.1021/bi00098a023.
7
Analysis of subsite preferences of HIV-1 proteinase using MA/CA junction peptides substituted at the P3-P1' positions.利用在P3 - P1'位置替换的MA/CA连接肽分析HIV-1蛋白酶的亚位点偏好性。
Arch Biochem Biophys. 1991 Oct;290(1):186-90. doi: 10.1016/0003-9861(91)90606-j.
8
HIV protease (HIV PR) inhibitor structure-activity-selectivity, and active site molecular modeling of high affinity Leu [CH(OH)CH2]Val modified viral and nonviral substrate analogs.HIV蛋白酶（HIV PR）抑制剂的结构-活性-选择性，以及高亲和力亮氨酸[CH(OH)CH2]缬氨酸修饰的病毒和非病毒底物类似物的活性位点分子建模。
Int J Pept Protein Res. 1992 Sep-Oct;40(3-4):274-81. doi: 10.1111/j.1399-3011.1992.tb00302.x.
9
A solid phase assay for the protease of human immunodeficiency virus.一种用于检测人类免疫缺陷病毒蛋白酶的固相分析方法。
Anal Biochem. 1990 Jul;188(1):82-5. doi: 10.1016/0003-2697(90)90530-m.
10
Mutational analysis of the substrate binding pocket of murine leukemia virus protease and comparison with human immunodeficiency virus proteases.小鼠白血病病毒蛋白酶底物结合口袋的突变分析及其与人类免疫缺陷病毒蛋白酶的比较。
J Biol Chem. 1995 Dec 8;270(49):29162-8. doi: 10.1074/jbc.270.49.29162.

引用本文的文献

1
New developments in solid phase synthesis supports.
Mol Divers. 1998;4(1):25-30. doi: 10.1023/a:1009636517017.
2
Predicting cleavability of peptide sequences by HIV protease via correlation-angle approach.
J Protein Chem. 1993 Jun;12(3):291-302. doi: 10.1007/BF01028191.