Board Philip G, Coggan Marjorie, Cappello Jean, Zhou Huina, Oakley Aaron J, Anders M W
Molecular Genetics Group, John Curtin School of Medical Research, Australian National University, Canberra, ACT 2601, Australia.
Anal Biochem. 2008 Mar 1;374(1):25-30. doi: 10.1016/j.ab.2007.09.029. Epub 2007 Sep 29.
Glutathione transferase omega 1-1 (GSTO1-1) catalyzes the biotransformation of arsenic and is implicated as a factor influencing the age-at-onset of Alzheimer's disease and the posttranslational activation of interleukin 1beta (IL-1beta). Investigation of the biological role of GSTO1-1 variants has been hampered by the lack of a specific assay for GSTO1-1 activity in tissue samples that contain other GSTs and other enzymes with similar catalytic specificities. Previous studies (P. G. Board and M. W. Anders, Chem. Res. Toxicol. 20 (2007) 149-154) have shown that GSTO1-1 catalyzes the reduction of S-(phenacyl)glutathiones to acetophenones. A new substrate, S-(4-nitrophenacyl)glutathione (4NPG), has been prepared and found to have a high turnover with GSTO1-1 but negligible activity with GSTO2-2 and other members of the glutathione transferase superfamily. A spectrophotometric assay with 4NPG as a substrate has been used to determine GSTO1-1 activity in several human breast cancer cell lines and in mouse liver and brain tissues.
谷胱甘肽转移酶ω1-1（GSTO1-1）催化砷的生物转化，并被认为是影响阿尔茨海默病发病年龄和白细胞介素1β（IL-1β）翻译后激活的一个因素。由于缺乏针对含有其他谷胱甘肽转移酶（GSTs）和具有相似催化特异性的其他酶的组织样本中GSTO1-1活性的特异性检测方法，对GSTO1-1变体生物学作用的研究受到了阻碍。先前的研究（P.G.博德和M.W.安德斯，《化学研究毒理学》20（2007）149 - 154）表明，GSTO1-1催化S-（苯甲酰甲基）谷胱甘肽还原为苯乙酮。一种新的底物S-（4-硝基苯甲酰甲基）谷胱甘肽（4NPG）已被制备出来，发现它与GSTO1-1反应的周转率很高，但与GSTO2-2及谷胱甘肽转移酶超家族的其他成员反应的活性可忽略不计。一种以4NPG作为底物的分光光度法已被用于测定几种人乳腺癌细胞系以及小鼠肝脏和脑组织中的GSTO1-1活性。
